Publication
Rhodol-based thallium sensors for cellular imaging of potassium channel
activity.
Authors Dutter BF, Ender A, Sulikowski GA, Weaver CD
Submitted By Submitted Externally on 8/20/2018
Status Published
Journal Organic & biomolecular chemistry
Year 2018
Date Published 8/1/2018
Volume : Pages 16 : 5575 - 5579
PubMed Reference 30051127
Abstract Thallium (Tl+) flux assays enable imaging of potassium (K+) channel activity in
cells and tissues by exploiting the permeability of K+ channels to Tl+ coupled
with a fluorescent Tl+ sensitive dye. Common Tl+ sensing dyes utilize
fluorescein as the fluorophore though fluorescein exhibits certain undesirable
properties in these assays including short excitation wavelengths and pH
sensitivity. To overcome these drawbacks, the replacement of fluorescein with
rhodols was investigated. A library of 13 rhodol-based Tl+ sensors was
synthesized and their properties and performance in Tl+ flux assays evaluated.
The dimethyl rhodol Tl+ sensor emerged as the best of the series and performed
comparably to fluorescein-based sensors while demonstrating greater pH tolerance
in the physiological range and excitation and emission spectra 30 nm red-shifted
from fluorescein.