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Experiment

Metabolic Clamp Study to Assess Insulin Sensitivity in HFD-Fed Mice Treated with
AAV-LbNOX
Summary Data Summary
Investigator Mootha, Vamsi
Description The background strain on a high-fat diet is a well-studied model of diet-induced
obesity with insulin resistance, and our transgene will allow us to study how
changes in NAD metabolism impact insulin resistance.

Applicable research area(s): Diabetes, Metabolism
Status Completed
Public Release 11/4/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals6
Experimental Conditions3
Catalog Items6
Curation Info (# flags)2
Phenotype Assays14
Phenotype Measurements84
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
6
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Drug Administered

 LbNOX
A water-forming NADH oxidase from Lactobacillus Brevis (Lb-NOX) is used as a genetic tool for inducing a compartment-specific increase of the NAD+/NADH ratio in human cells.
 Luciferase
An enzyme that catalyzes the oxidation of luciferin and ATP, producing light.

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Teklad - 93075
Harlan-Teklad TD.93075 Adjusted Calorie diet (Calories: 21% protein, 55% Fat, 24% Carbohydrate) A diet used to induce obesity. Approximate fatty acid profile (% total fat): 28% saturated, 30% trans, 28% monounsaturated (cis), 14% polyunsaturated...



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