University of California Davis
University of Cincinnati Medical Center
University of Massachusetts Medical School
University of Michigan Medical School
Vanderbilt University School of Medicine
Protocols & Methods
Reagents & Resources
Tissues & Samples
Conditions of Use
Energy Expenditure Analysis
Guidelines & Policies
Rhodol-based thallium sensors for cellular imaging of potassium channel
Dutter BF, Ender A, Sulikowski GA, Weaver CD
Submitted Externally on 8/20/2018
Organic & biomolecular chemistry
Volume : Pages
16 : 5575 - 5579
Thallium (Tl+) flux assays enable imaging of potassium (K+) channel activity in
cells and tissues by exploiting the permeability of K+ channels to Tl+ coupled
with a fluorescent Tl+ sensitive dye. Common Tl+ sensing dyes utilize
fluorescein as the fluorophore though fluorescein exhibits certain undesirable
properties in these assays including short excitation wavelengths and pH
sensitivity. To overcome these drawbacks, the replacement of fluorescein with
rhodols was investigated. A library of 13 rhodol-based Tl+ sensors was
synthesized and their properties and performance in Tl+ flux assays evaluated.
The dimethyl rhodol Tl+ sensor emerged as the best of the series and performed
comparably to fluorescein-based sensors while demonstrating greater pH tolerance
in the physiological range and excitation and emission spectra 30 nm red-shifted
Back to Top
There was a problem with the page:
Safari Browser Detected...
We strive to make the DiaComp site compatable with as many browsers as possible, but some of our third party tools don't work with the Safari browser.
In order to explore this site we highly recommend using the most recent versions of the following browsers:
The MMPC is a National Institutes of Health-sponsored resource that provides experimental testing services to scientists studying diabetes, obesity, diabetic complications, and other metabolic diseases in mice.
Interested in receiving MMPC News?
2017 National MMPC. All Rights Reserved.