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Rhodol-based thallium sensors for cellular imaging of potassium channel
Dutter BF, Ender A, Sulikowski GA, Weaver CD
Submitted Externally on 8/20/2018
Organic & biomolecular chemistry
Volume : Pages
16 : 5575 - 5579
Thallium (Tl+) flux assays enable imaging of potassium (K+) channel activity in
cells and tissues by exploiting the permeability of K+ channels to Tl+ coupled
with a fluorescent Tl+ sensitive dye. Common Tl+ sensing dyes utilize
fluorescein as the fluorophore though fluorescein exhibits certain undesirable
properties in these assays including short excitation wavelengths and pH
sensitivity. To overcome these drawbacks, the replacement of fluorescein with
rhodols was investigated. A library of 13 rhodol-based Tl+ sensors was
synthesized and their properties and performance in Tl+ flux assays evaluated.
The dimethyl rhodol Tl+ sensor emerged as the best of the series and performed
comparably to fluorescein-based sensors while demonstrating greater pH tolerance
in the physiological range and excitation and emission spectra 30 nm red-shifted
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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