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Full-length mouse Tnmd with a C-terminal Flag tag was
inserted 3' to 5.4-kb adiponectin promoter at the Cla I
site69. After verifying both ends by sequencing, the
transgenic cassette was linearized by Kpn1 and Xho1
digestion, purified and submitted for pro-nuclear injection.
The transgene was introduced into embryos from C57BL/6J mice
(000058; Jackson Laboratories). Embryos were then implanted
into pseudopregnant C57BL6/J females by the UMASS Transgenic
Animal Facility. Male transgenic animals were crossed with
C57BL6/J females. Genotyping was performed by PCR from
genomic DNA with the following primers: 5'-
GACCAGAATGAGCAATGGGTG , 3'- ATCGTCGTCATCCTTGTAGTCG -3'.
Genomics - Transgenes
University of Massachusetts Medical School
Curation Flag Information
New comment to be added:
Metabolic Cage and Clamp Study in HFD-Fed p5.4-TNMD Mice
Metabolic Cage Study in HFD-fed CD40 KO Mice
Metabolic Clamp Study in HFD-fed p5.4-TNMD-Flag Mice (Batch 2)
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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