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Catalog Item

Hyperinsulinemic clamp

Common Name(s): Hyperinsulinemic-euglycemic clamp

Center	Vanderbilt University School of Medicine
Center Core	Metabolic Regulation Core
Test No.	V3005
Test Price	$802/mouse with 6,6D-Glucose + 14C-2Deoxyglucose (includes surgery, full analysis, and insulin levels)
Description	The hyperinsulinemic clamp is used to measure insulin action in vivo. Hyperinsulinemic clamps are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. A continuous infusion of insulin is given. Glucose levels are monitored in arterial samples every 5-10 min using an Accuchek glucose analyzer that allows the analysis of glucose with only 1 µl of blood. Glucose is infused in the jugular vein catheter at rates necessary to achieve the desired glucose level, based on feedback from arterial glucose measurements. These methods allow assessment of the responsiveness of the body to insulin. Blood from a donor animal is infused to maintain blood volume. By combining this technique with the tracer method one can also examine the impact of insulin on suppression of endogenous glucose production. (1, 2) 1. Ayala JE, Bracy DP, Malabanan C, James FD, Ansari T, Fueger PT, et al. Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice. J Vis Exp. 2011:e3188. 2. Halseth AE, Bracy DP, Wasserman DH. Overexpression of hexokinase II increases insulin and exercise-stimulated muscle glucose uptake in vivo. Am J Physiol. 1999 1/1999;276(1 Pt 1):E70-E7.
Data	Data will be collected for this catalog item
Keywords	clamp, diabetes, endogenous glucose production, glucose, glucose disposal, glucose uptake, hyperinsulinemic clamp, insulin, insulin action, insulin resistance
Tissues	adipose tissue, brown adipose tissue, heart, liver, skeletal muscle

Name	Options
Carbohydrate Metabolism
Diabetes
Insulin and Insulin Function
Liver Function
Endocrine Deficiency
Surgery
Metabolism and Metabolomics
Obesity

Name	Abbreviation	Options
glucose, blood	blood glucose
body mass	W rodent
hematocrit, arterial	None
glucose rate of appearance, endogenous	endo RA
glucose, rate of disappearance	Rd
Kg, vastus lateralis	None
Rg, brain	None
Rg, gastrocnemeous	None
Rg, heart	None
Rg, soleus	None
Rg, vastus lateralis	None
Kg, brain	None
Kg, gastrocnemius	None
Kg, heart	None
Kg, soleus	None
insulin, plasma, arterial	None

Name	Units	Description	Abbreviation	Min Value	Max Value	Categorical	Batch	Options
Mouse Diet		This is the food/chow mice were fed during the experiment.		-	-	True	False
Time Since Surgery (day)	day	The time, in days, since the surgery was performed.		-	-	False	True
Time Sample Taken	minute	Time in minutes since a sample was taken		-	-	False	True
Insulin Infusion Rate - milliUnits	milliUnits per kilogram per minute	This is insulin infusion rate for hyperinsulinemic clamp data		0	-	False	False
Fast Duration	hour	Length of time the animal was fasted	fast duration	0	24	False	False
Drug Administered		The drug that was administered during the study.		-	-	True	False
Drug Dose	arbitrary units	A numerical value indicating the dose of a drug administered. The unit of measure is indicated in the Drug Dose Units categorical value that accompanies this experimental condition.	Drug Dose	0	-	False	False
Surgical Procedure		The surgical procedure used for this experiment.	Surgical Procedure	0	0	True	False
Drug Dose Units		The units used for the drug dose experimental factor	Drug Dose Units	0	0	True	False

Title	Type	Version	Investigator(s)	Inactive	Options
Vandy – Chronic Catheterization of carotid artery and jugular vein		1	Lantier, Louise	Active
Vandy - Hyperinsulinemic-euglycemic Clamp		1		Active
Vandy - Hyperinsulinemic-Hypoglycemic clamp		1		Active
Vandy – Post Clamp Anesthesia		1	Lantier, Louise	Active

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