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Protocols

The following protocols are used by the MMPC. Click the title for details, or download the protocol in PDF format (if available).


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 TitleVersionEducationOptions

Type: Breeding

 117NA UC Davis - Acquisition, Acclimation, Quarantine1Active
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 374NA UC Davis - Colony Management1Active
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 375NA UC Davis - Conventional Per Diem1Active
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 266NA UC Davis - High fat diet feeding2Active
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 119NA UC Davis - Husbandry Care for Mice1Active
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 261NA UC Davis - Mouse Model Creation1Active
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 118NA UC Davis - Receipt of Vendor and Courier Mice1Active
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Type: Histology

 317NA U Michigan - Cryoembedding1Active
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Type: Phenotype Assay

 304NA U Michigan - DNA Extraction for Illumina 16S rRNA Extraction1Active
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 310NA U Michigan - Glomerular Filtration Rate determination with minipump inulin clearance1Active
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 318NA U Michigan - Hind Paw Withdrawal for Rodents1Active
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 305NA U Michigan - Illumina 16S rRNA gene sequencing using DNA1Active
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 319NA U Michigan - Intra-Epidermal fiber Density1Active
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 312NA U Michigan - Mesangial matrix evalutaion1Active
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 320NA U Michigan - Nerve Conduction Velocity1Active
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 306NA U Michigan - Optokinetic Measurements of Visual Acuity and Contrast Sensitivity1Active
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 307NA U Michigan - Retinal Cell Death1Active
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 308NA U Michigan - Retinal Microstructure Imaging OCT1Active
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 309NA U Michigan - Retinal Vascular Permeability1Active
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 314NA U Michigan - Spot urine collection1Active
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 315NA U Michigan - Tail cuff Blood Pressure determination1Active
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 321NA U Michigan - Thiobarbituric acid reactive substances (TBARS)1Active
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 359NA UC Davis - 3-chamber Sociability & Social Novelty test1Active
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 284EE/shared/rigor.aspx?webinar=EEUC Davis - Adiposity1ActiveWebinar Available
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 372NA UC Davis - Analysis of Metabolites from Tissue, Feces or Other Fluids by Mass Spectrometry1Active
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 358NA UC Davis - Auditory startle & Prepulse inhibition1Active
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 357NA UC Davis - Blood Pressure1Active
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 371NA UC Davis - Body Composition Analysis by EchoMRI1Active
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 367NA UC Davis - Calprotectin ELISA1Active
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 370NA UC Davis - CCK Inhibition of Diet Intake1Active
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 92NA UC Davis - Cholesterol Protocol1Active
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 285EE/shared/rigor.aspx?webinar=EEUC Davis - CLAMS Meal Pattern Analysis1ActiveWebinar Available
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 356NA UC Davis - Clinical Blood Chemistry1Active
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 246EE/shared/rigor.aspx?webinar=EEUC Davis - Comprehensive Laboratory Animal Monitoring System (CLAMS)3ActiveWebinar Available
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 265NA UC Davis - Corticosterone RIA1Active
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 286EE/shared/rigor.aspx?webinar=EEUC Davis - Digestible Energy by Bomb Calorimetry1ActiveWebinar Available
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 115NA UC Davis - Echocardiography1Active
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 114NA UC Davis - Electrocardiography1Active
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 354NA UC Davis - Elevated Plus Maze1Active
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 96NA UC Davis - ELISA Protocol1Active
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 300NA UC Davis - Ex vivo assessment of barrier function-gut permeability1Active
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 353NA UC Davis - Fear Conditioning1Active
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 352NA UC Davis - Fecal Collection1Active
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 364NA UC Davis - Feces Collection for Gut Microbiome Analysis1Active
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 337NA UC Davis - Functional Observational Battery1Active
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 351NA UC Davis - Gait Analysis1Active
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 260NA UC Davis - Genotyping2Active
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 360NA UC Davis - Glucagon ELISA1Active
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 350NA UC Davis - Grip Strength Test1Active
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 104NA UC Davis - Gross Body Composition (DEXA)1Active
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 349NA UC Davis - Hargreaves-Nociception Test1Active
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 363NA UC Davis - HDL-TG and LDL-TG/VLDL-TG1Active
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 348NA UC Davis - Hematology1Active
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 365NA UC Davis - Hemoglobin A1C1Active
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 373NA UC Davis - Hepatic Triglyceride Production1Active
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 245NA UC Davis - Imaging CMGI1Active
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 347NA UC Davis - In Vivo Gut Permeability1Active
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 86NA UC Davis - IN-VIVO Glucose-stimulates Insulin Secretion Test1Active
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 346NA UC Davis - Intestinal Permeability1Active
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 85NA UC Davis - Intraperitoneal Glucose Tolerance Test1Active
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 84NA UC Davis - Intraperitoneal Insulin Tolerance Test1Active
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 344NA UC Davis - Light-Dark Test1Active
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 368NA UC Davis - Lipocalin-2 ELISA1Active
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 299NA UC Davis - Lipoprotein Binding Protein (LBP)-Endotoxemia Assay1Active
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 343NA UC Davis - Marbles Burying Test1Active
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 369NA UC Davis - Meal Pattern Analysis and Diet/Taste Preference1Active
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 278NA UC Davis - Morris Water Maze1Active
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 95NA UC Davis - Non-esterified fatty acids Protocol1Active
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 342NA UC Davis - Novel Object Recognition Test1Active
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 341NA UC Davis - Open Field Test1Active
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 362NA UC Davis - Oral Glucose Tolerance Test1Active
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 277NA UC Davis - Radial Arm Water Maze1Active
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 340NA UC Davis - Rotarod1Active
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 301EE/shared/rigor.aspx?webinar=EEUC Davis - Running Wheel1ActiveWebinar Available
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 90NA UC Davis - ß hydroxy butyrate Protocol1Active
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 361NA UC Davis - Total Cholesterol (TC)1Active
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 339NA UC Davis - Touchscreen Operant Testing1Active
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 91NA UC Davis - Triglyceride Protocol1Active
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 336NA UC Davis - Urine Collection1Active
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 338NA UC Davis - Von Frey-Nociception Test1Active
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 345NA UC Davis - Whole Intestinal & Colonic Transit Time1Active
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 279NA UC Davis - Y-Maze1Active
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 237EE/shared/rigor.aspx?webinar=EEVandy - Exercise Stress Test1ActiveWebinar Available
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 240NA Vandy - Hyperglycemic clamp1Active
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 239NA Vandy - Hyperinsulinemic-euglycemic Clamp1Active
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 235NA Vandy - Hyperinsulinemic-Hypoglycemic clamp1Active
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 229NA Vandy - Mouse Blood Pressure Telemetry1Active
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 225NA Vandy - Mouse Echocardiography1Active
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 227NA Vandy - Mouse Myocardial Infarction1Active
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 226NA Vandy - Myocardial Ischemia Reperfusion1Active
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 224NA Vandy - Tail-Cuff Blood Pressure1Active
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 228NA Vandy - Transverse Aortic Constriction1Active
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 296EE/shared/rigor.aspx?webinar=EEVandy – Body Composition1ActiveWebinar Available
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 241EE/shared/rigor.aspx?webinar=EEVandy – Energy Balance with Promethion1ActiveWebinar Available
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 238NA Vandy – IP glucose tolerance test1Active
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 298NA Vandy – Oral glucose tolerance test1Active
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 206NA Yale - Alanine Aminotransferase1Active
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 208NA Yale - Alkaline Phosphatase1Active
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 207NA Yale - Aspartate Aminotransferase1Active
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 215EE/shared/rigor.aspx?webinar=EEYale - Beta hydroxybutyrate (Cobas)1ActiveWebinar Available
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 205NA Yale - Blood Albumin1Active
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 204NA Yale - Blood and Urine Creatinine1Active
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 217NA Yale - Blood and Urine Inorganic Phosphorous1Active
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 202NA Yale - Blood Glucose1Active
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 216NA Yale - Blood or Urine Calcium1Active
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 203NA Yale - Blood Urea Nitrogen1Active
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 212NA Yale - Cholesterol1Active
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 219NA Yale - Creatine Kinase Activity1Active
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 210NA Yale - HDL Cholesterol1Active
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 211NA Yale - LDL Cholesterol1Active
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 218NA Yale - Magnesium1Active
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 214NA Yale - Non-Esterified Fatty Acids1Active
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 201NA Yale - Tissue Glycogen1Active
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 209NA Yale - Total Protein1Active
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 213NA Yale - Triglycerides1Active
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Type: Staining

 311NA U Michigan - Massons Trichrome staining1Active
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 313NA U Michigan - Sirius Red staining1Active
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Type: Technique

 316NA U Michigan - Western blot1Active
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 366NA UC Davis - Preparation of Fecal Extracts for ELISAs1Active
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 292NA Vandy - Biliopancreatic Diversion in Mice1Active
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 294NA Vandy - Modified Roux-en-Y Gastric Bypass in Mice1Active
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 295NA Vandy - Vertical Sleeve Gastrectomy in Mice1Active
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 293NA Vandy – Bile Diversion in Mice1Active
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 236NA Vandy – Chronic Catheterization of carotid artery and jugular vein1Active
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 297NA Vandy – Post Clamp Anesthesia1Active
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 24NA Vascular perfusion of mice1Active
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