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Catalog Item
Hyperglycemic clamp
Common Name(s): Hyperglycemic clamp

CenterVanderbilt University School of Medicine
Center CoreMetabolic Regulation Core
Test No.V3006
Test Price$610/mouse with Insulin and c-Peptide (includes surgery and analysis)
DescriptionThe responsiveness of the pancreas to glucose is assessed using the
hyperglycemic clamp. Hyperglycemic clamps are performed on conscious mice with
catheters chronically implanted in the jugular vein and carotid artery. Our
clamps include 11 arterial insulin measurements and 3 arterial c-peptide
measures.
A defined hyperglycemic stimulus is created using a primed variable glucose
infusion to raise the glucose level to twice basal for 120 min. An established
priming algorithm is used to elevate glucose quickly.
Mice with extra copies of the glucokinase gene locus were demonstrated to have a
blunted insulin response to hyperglycemia using this technique (Niswender et al.
J. Biol. Chem. 1997).
DataData will be collected for this catalog item
Keywordsclamp, diabetes, glucose, hyperglycemic clamp, insulin, insulin resistance,
insulin secretion, insulin sensitivity
Tissuespancreas



Catalog Groups



Phenotype Assays



Experimental Factors
NameUnitsDescriptionAbbreviationMin ValueMax ValueCategoricalBatchOptions
Mouse Diet 
This is the food/chow mice were fed during the experiment.
 --TrueFalse
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Time Since Surgery (day)day
The time, in days, since the surgery was performed.
 --FalseTrue
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Time Sample Takenminute
Time in minutes since a sample was taken
 --FalseTrue
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Fast Durationhour
Length of time the animal was fasted
fast duration024FalseFalse
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Drug Administered 
The drug that was administered during the study.
 --TrueFalse
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Drug Dosearbitrary units
A numerical value indicating the dose of a drug administered. The unit of measure is indicated in the Drug Dose Units categorical value that accompanies this experimental condition.
Drug Dose0-FalseFalse
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Surgical Procedure 
The surgical procedure used for this experiment.
Surgical Procedure00TrueFalse
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Drug Dose Units 
The units used for the drug dose experimental factor
Drug Dose Units00TrueFalse
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