University of California Davis
University of Cincinnati Medical Center
University of Massachusetts Medical School
University of Michigan Medical School
Vanderbilt University School of Medicine
Protocols & Methods
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Tissues & Samples
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Tracers in Metabolic Research
Isotope Tracers in Metabolic Research: 3-Part Webinar Series
Energy Expenditure Analysis
CalR: Indirect Calorimetry Analysis
Guidelines & Policies
New comment to be added:
Effect of Heph (hephaestin) ablation on energy expenditure and body composition
Hephaestin is a protein expressed in many tissues in the body that are involved
in iron export from cells. Mice with a mutation in this gene have been shown to
accumulate iron in the brain and intestinal cells. We have preliminary evidence
to suggest an association with weight gain. Mutant mice also exhibit motor
defects with age.
Applicable research area(s): Obesity, Metabolism, Neurobiology
week(s) post-natal (w)
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Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor: Mouse Diet
Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...
Experimental Factor (Units)
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energy intake (24 hr)
energy intake (24 hr)
oxygen consumption (Light Period)
fat body mass
fat body mass
lean body mass
lean body mass
respiratory exchange ratio (Light Period)
bone mineral density
bone mineral content
fat tissue, %
% body fat
heat (Light Period)
oxygen consumption (Dark Period)
carbon dioxide production Light Period
carbon dioxide production Dark Period
respiratory exchange ratio (Dark Period)
heat (Dark Period)
carbon dioxide production
respiratory exchange ratio
energy intake (Light Period)
food intake - light
food intake (Dark Period)
food intake - dark
energy intake (avg 24 hr)
energy intake avg 24
energy intake (Ligth Period)
energy intake -light
energy intake (Dark Period)
energy intake - dark
Activity 24h (total x-axis)
Activity 24h total x
Activity 24h (x-ambulatory)
Activity 24h (x-amb)
Activity 24h (total vertical z-axis)
Activity 24h total z
total tissue mass
heat - 24h
meal duration (Avg) - Active Eating Time
avg meal dur AET
meal size (Avg)
avg meal size
inter-meal interval, average
meal duration (total)
total meal duration
eating rate, average
avg eating rate
meal duration, average light
avg light-meal dur
meal duration, average dark
avg dark-meal dur
meal size, average light
avg light-meal size
meal size, average dark
avg dark-meal size
inter-meal interval, average light
inter-meal interval, average dark
meals, number light
meals, number dark
satiety ratio, light
satiety ratio, dark
eating rate, light
eating rate, dark
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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