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Analytical Study 3 in Astellas-Treated NZBWF1/j Mice
Summary Data Summary
Investigator Yavanian, Gregory
Description NZBWF1/j mice contain a natural genetic makeup that is prone to lupus related
autoimmunity. They can be used to study effects of lupus nephritis, including
elevated anti-nuclear antibodies, metabolites, as well as kidney disease
Status Completed
Public Release 9/6/2020
Animal Age Measured In: week(s) post-natal (w)
Flags No data
Data Analysis
Experimental Conditions2
Catalog Items2
Curation Info (# flags)1
Phenotype Assays2
Phenotype Measurements0
Histology Images0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 No Drug/Treatment
No Drug or Treatment done on these samples
Lipopolysaccharide - induces bacterial infection
Human Recombinant Hyduronidase P.H. 20 Drug Dose is in mg/kg body weight
10 mM Histidine, 130 mM NaCL at P.H. 6.5
REGN379 - Proprietary drug formulation
REGN379(aDll4) - Proprietary drug formulation
Leptin administered
 Water with 7,8 Dihydrofolate
Water with 7,8 Dihydrofolate
 Leptin/7,8 Dihydrofolate
Animal had both Leptin and Water with 7,8 Dihydrofolate.
 CD4 AT Cells
Adoptive Transfer of CD4 T cells by Tail Vein Injections.
 CD8 AT Cells
Adoptive transfer of CD8 T cells by Tail Vein Injections.
N-acyl phosphatidylethanolamines (NAPEs).
Cloned Arabidopsis thaliana NAPE synthase (pNAPES).
E. coli Nissle 1917 (EcN).
pEcN is EcN with empty plasmid without NAPE as a control.
Vehicle - Control
PI3K inhibitor - medical drug that functions by inhibiting one or more of the phosphoinositide 3-kinase enzymes
Over-expression of sortilin 1 protein
Both PI3K inhibitor (medical drug that functions by inhibiting one or more of the phosphoinositide 3-kinase enzymes) and over-expression of sortilin 1 protein.
Salicylamine added to the drinking water at a concentration of 3g/L.
Control anti-sense oligonucleotide
Target anti-sense oligonucleotide (ASO)-1
Target anti-sense oligonucleotide (ASO)-2
Control humanized antibody
Humanized bispecific antibody that selectively activates FGFR1/ß Klotho complex
Octreotide (brand name Sandostatin, Novartis Pharmaceuticals) is an octapeptide that mimics natural somatostatin pharmacologically, though it is a more potent inhibitor of growth hormone, glucagon, and insulin than the natural hormone
Pasireotide (SOM230, trade name Signifor) is an orphan drug approved in the U.S. and Europe for the treatment of Cushing's disease in patients who fail or are ineligible for surgical therapy. It was developed by Novartis. Pasireotide is a...
SHIP1 Inhibitor. SH2-Domain containing inositol 5-phosphatases (SHIP1 & SHIP2) dephosphorylate the 5-position of PI(3,4,5)P3 generating PI(3,4)P2. SHIP2 is ubiquitously expressed while SHIP1 is only found in hematopoietic lineage cells. 3AC is a...
3AC derivative also known as Soluable 3AC
Suberoylanilide hydroxamic acid - a histone deacetylase (HDAC) inhibitor
Tacedinaline - a histone deacetylase (HDAC) inhibitor
Polyethylene glycol cystathionine beta-synthase
 Water with PCA
Water with penicillamine
Stichodactyla toxin that is a selective and potent blocker of the voltage-gated Kv1.3 channel
pCMV6 plasmid encoding for murine FGFBP3
pCMV6 empty plasmid (control)
pCMV6 plasmid encoding for murine FGFBP1
Metformin is a drug approved by the U.S. Food and Drug Administration as a prescription medication to treat diabetes. This medication is used to decrease hepatic (liver) glucose production, to decrease GI glucose absorption and to increase target...
 Peptide - Control
Control peptide
 Peptide - RGD
RGD Peptide
Centrally acting anti-inflammatory drugs for the treatment of Frontotemporal Dementia (FTD). These compounds are orally active. Their mechanism of action is inhibition of tumor necrosis factor-alpha (TNFa) via blockade of TNFa synthesis by...
Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure. It is part of the renin-angiotensin system, which is a major target for drugs that lower blood pressure. Angiotensin also stimulates the...
E. coli Nissle 1917 expressing pNAE to increase production of N-acylethanolamide (NAE) family of lipids
 shRNA - Becn1
shRNA-mediated knockdown of Beclin1 (Becn1)
7,8-Dihydroxyflavone (7,8-DHF) is a naturally-occurring flavone found in Godmania aesculifolia, Tridax procumbens, and primula tree leaves. It has been found to act as a potent and selective small-molecule agonist of the TrkB receptor (Kd ˜ 320...
A salt or ester of acetic acid.
Glycerol (also called glycerine or glycerin) is a simple polyol (sugar alcohol) compound.
Animals received both Acetate and Glycerol
Inhibitor of the Raf-activated MAP/ERK kinase (MEK), Barrett et al. Bioorganic & Medicinal Chemistry Letters 2008
A PPARg antagonist. (S)-4'-((5-((1-(4-nitrophenyl)ethyl)carbamoyl)-1H-indol-1-yl)methyl)-[1,1'-biphenyl]-2-carboxylic acid.
A combination of ciprofloxacin with an antimicrobial agent active against anaerobes, such as metronidazole, treats a mixed aerobic/anaerobic infections.
HIF prolyl-4-hydroxylase inhibitor
Combination of N-arachidonoylethanolamine and palmitoylethanolamide
Imeglimin is an experimental drug being developed as an oral anti-diabetic. It is an oxidative phosphorylation blocker that acts to inhibit hepatic gluconeogenesis, increase muscle glucose uptake, and restore normal insulin secretion.
Fibroblast growth factor 21 is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family and specifically a member of the "endocrine" subfamily which includes...
oleoylethanolamine and palmitoylethanolamide
 HDAC inhibitor
histone deacetylase inhibitor
Digoxin is a purified cardiac glycoside similar to digitoxin extracted from the foxglove plant, Digitalis lanata. Digoxin is occasionally used in the treatment of various heart conditions, namely atrial fibrillation, atrial flutter and sometimes...
niclosamide ethanolamine salt
HIF prolyl-hydroxylase inhibitors are members of a class of new drugs that act on the hypoxia-inducible factor (HIF) pathway.
 AgRP Peptide
Agouti-related peptide(AgRP) is a neuropeptide produced in the brain by the AgRP/NPY neuron. AgRP is a paracrine signalling molecule made up of 112 amino acids (the gene product of 132 amino acids is processed by removal of the N-terminal...
Didox is a simple, synthetic antioxidant that has been found to reduce the levels of oxidative injury markers in the brains of HIV patients with dementia
Doxorubicin is an anthracycline antitumor antibiotic that inhibits DNA topoisomerase II by inducing double-stranded DNA breaks.
Fibroblast growth factor 19 is a protein that in humans is encoded by the FGF19 gene. It functions as a hormone, regulating bile acid synthesis, with effects on glucose and lipid metabolism
Flavopiridol hydrochloride is a potent CDK (cyclin-dependent kinase), and a CDC25 phosphatase family inhibitor.
JQ1 is a thienotriazolodiazepine and a potent inhibitor of the BET family of bromodomain proteins
The drug that was administered during the study.
Sirolimus, also known as rapamycin, is a macrolide compound that has immunosuppressant functions in humans and is especially useful in preventing the rejection of kidney transplants. It inhibits activation of T cells and B cells by reducing their sensitiv
 NV compound
The drug that was administered during the study.
insulin sensitizer
The drug that was administered during the study.
The drug that was administered during the study.
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs.
It can treat high cholesterol and triglyceride levels. This may reduce the risk of angina, stroke, heart attack, and heart and blood vessel problems.
It can treat fluid retention (edema) and swelling caused by congestive heart failure, liver disease, kidney disease, and other medical conditions.
specific BTK inhibitor
Valproic acid (VPA) can treat seizures and bipolar disorder. It can also help prevent migraine headaches.
Berberine is a chemical found in several plants including European barberry, goldenseal, goldthread, Oregon grape, phellodendron, and tree tumeric. People take berberine for heart failure.
 Concanavalin A
Induces hepatitis
 Olive Oil
The drug that was administered during the study.
VEGF blocker
The drug that was administered during the study.
oral vitamin D3 supplement
Carrageenan was added to the drinking water. Carrageenan are a family of linear sulfated polysaccharides that are extracted from red edible seaweeds. They are widely used in the food industry, for their gelling, thickening, and stabilizing properties. Th
DDT is a synthetic organic compound used as an insecticide. Like other chlorinated aromatic hydrocarbons, DDT tends to persist in the environment and become concentrated in animals at the head of the food chain.
Olive oil vehicle
experimental oligonucleotide that destabilizes an mRNA called ApoC3 that affects serum triglyceride clearance
The drug that was administered during the study.
The drug that was administered during the study.
Insulin-Degrading Enzyme (IDE) Compound A - Saghatelian, Alan
Insulin-Degrading Enzyme (IDE) Compound B - Saghatelian, Alan
Insulin-Degrading Enzyme (IDE) Compound C - Saghatelian, Alan
Bone morphogenetic protein - induce the formation of bone and cartilage, orchestrate tissue architecture throughout the body. BMP-7 has recently found use in the treatment of chronic kidney disease (CKD) and has been shown in murine animal models...
 Cholic acid
Cholic acid is a bile acid - aids in digestion of fats and certain nutrients.
Proprietary compound from Navitor
 MC Tween
Chemical buffer
Formestane (trade name Lentaron), also known as 4-hydroxyandrost-4-ene-3,17-dione, is a type I, steroidal, selective aromatase inhibitor. It is used in the treatment of estrogen receptor-positive breast cancer in postmenopausal women. The drug is...
Proprietary compound from Metacrine
Proprietary compound from UCB
 Cholesterol conjugated hsiRNA
Cholesterol was conjugated to hsiRNA (heterochromatic short interfering RNA).
Premarin is the brand name for an estrogen medication that consists of conjugated estrogens.
Phosphate-buffered saline is a buffer salt solution containing disodium hydrogen phosphate and sodium chloride.
Letrozole (INN, trade name Femara) is an oral non-steroidal aromatase inhibitor for the treatment of hormonally-responsive breast cancer after surgery. These are continuous release pellets.
AM-6545 is a drug which acts as a peripherally selective silent antagonist for the CB1 receptor and was developed for the treatment of obesity.
 IL-5, recombinant
Interleukin 5 (IL-5) is an interleukin produced by type-2 T helper cells and mast cells. This is the recombinant form of IL-5
 Ampicillin, Neomycin, Vancomycin
1g/L of Ampicillin, 1g/L of Neomycin, and 0.5g/L of Vancomycin mixed in water
 CL 316,243
A highly selective ß3 adrenoceptor agonist that has been shown to cause increases in brown adipose tissue thermogenesis and metabolic rate as well as decreases in blood insulin and glucose levels.
Proprietary compound from 1GHI.
Proprietary compound from Astellas
Proprietary compound from Imagine
siRNA targeting luciferase
The drug that was administered during the study.
A water-forming NADH oxidase from Lactobacillus Brevis (Lb-NOX) is used as a genetic tool for inducing a compartment-specific increase of the NAD+/NADH ratio in human cells.
An enzyme that catalyzes the oxidation of luciferin and ATP, producing light.
anti-gonococcal therapeutic (CMP-nonulosonate)
 Compound 1
siRNA targeting proprietary gene of interest, from the lab of Ken Fujise - University of Cincinnati Medical Center client.
MB1-47, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB1-78, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB1-98, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB1-103, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB1-105, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB5-1, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
MB5-6, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
2'-Fucosyllactose is an oligosaccharide. It is the most prevalent human milk oligosaccharide (HMO), making up about 30% of all of HMOs.
Prophorce SR710 - Gut health and performance enhancer based on monobutyrin
Y05, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
WX06, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 Asprosin 1
Asprosin is a protein hormone produced by mammals in their fatty (white adipose) tissues during fasting that stimulates the liver to release glucose into the blood stream - isoform 1
 Asprosin 2
Asprosin is a protein hormone produced by mammals in their fatty (white adipose) tissues during fasting that stimulates the liver to release glucose into the blood stream - isoform 2
 Asprosin 3
Asprosin is a protein hormone produced by mammals in their fatty (white adipose) tissues during fasting that stimulates the liver to release glucose into the blood stream - isoform 3
Carbon tetrachloride used for inducing liver fibrosis
Exendin-4 is an incretin mimetic peptide, which is composed of 39 amino acids. It is an analog of glucagon-like peptide 1 (GLP-1), and an insulinotropic agent, with a long half-life. Exendin-4 has been used for the treatment of ob/ob mouse model t...
IgG2b isotype mouse monoclonal antibody against avß5; stock concentration 1.9 mg/ml
 2H6-2C IGg2B
control antibody (for comparison to ALULA); stock concentration 2.2 mg/ml
Semisynthetic broad-spectrum cephalosporin with a tetrazolyl moiety that is resistant to beta-lactamase. It has been proposed especially against Pseudomonas infections.
Vancomycin is used to treat colitis (inflammation of the intestine caused by certain bacteria) that may occur after antibiotic treatment. Vancomycin is in a class of medications called glycopeptide antibiotics. It works by killling bacteria in the...
Neomycin is an aminoglycoside antibiotic found in many topical medications such as creams, ointments, and eyedrops.
 Vancomycin, Neomycin
0.5 g/L Vancomycin and 1.0 g/L Neomycin combination
A metallic element that forms a number of poisonous compounds
cocktail of 0.5g/L Vancomycin and 1.0g/L Neomycin .
Estradiol (E2), also spelled oestradiol, is an estrogen steroid hormone and the major female sex hormone. It is involved in the regulation of the estrous and menstrual female reproductive cycles.
GABA (Gamma-Aminobutyric Acid) is the chief inhibitory neurotransmitter in the mammalian central nervous system. Its principal role is reducing neuronal excitability throughout the nervous system. In humans, GABA is also directly responsible for...
Proprietary compound from Astellas.
 Txnip Inhibitor
Thioredoxin-interacting protein inhibitor
chemotherapy medication used to treat a number of cancers
Streptozotocin (STZ) is an alkylating agent that targets insulin-producing beta cells in the pancreas, mimicking Type 1 diabetes phenotypes in mice.
Growth Differentiation Factor 11 - This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family t
RNA interference targeting the Agrp gene
 Maltose dextrin
Maltodextrin is a polysaccharide that is used as a food additive.
N-acetyl-para-aminophenol (APAP),Tylenol, Panadol. It belongs to a class of drugs called analgesics and antipyretics.
WX53, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
The drug that was administered during the study.
Ohio University custom compound 1, inhibitor of inflammation. The compound is proprietary to Ohio University.
Ohio University custom compound 2, inhibitor of inflammation. The compound is proprietary to Ohio University.
 Soluble epoxide hydrolase (sEH) inhibitor
Soluble epoxide hydrolase (sEH) is an enzyme that catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), which are lipid mediators derived from arachidonic acid through the cytochrome P450 epoxygenase pathway. Inhibitors sEH appear to reduce the
TPPU (N-[1-(1-oxopropyl)-4-piperidinyl]-N-[4-(trifluoromethoxy)phenyl)-urea) is a potent inhibitor of both human and mouse sEH.
Y03, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution.
CRISPR-delivery particles (CriPs), composed of nano-size complexes of Cas9 protein and sgRNA that are coated with an amphipathic peptide called Endo-Porter that mediates entry into cells.
ULM compound, hypocholesterolemic class.The compound is proprietary to ULM.
The compound is proprietary to Hangzhou Just Biotherapeutics.
Human milk oligosaccharides
Hepatic specific GABA- transaminase (ABAT) anti-sense oligonucleotide (ASO).
regulates immunity response
 rat IgG
rat immunoglobulin antibody
antibody to NK1.1
antibody to ASGM1
antibody to PD-1
JR-AB2-011 is a selective mTORC2 inhibitor with an IC50 value of 0.36 µM. JR-AB2-011 inhibits mTORC2 activity by blocking Rictor-mTOR association (Ki: 0.19 µM). JR-AB2-011 has anti-glioblastoma multiforme (GBM) properties
Torkinib (PP242) is a selective mTOR inhibitor with IC50 of 8 nM in cell-free assays; targets both mTOR complexes with >10- and 100-fold selectivity for mTOR than PI3Kd or PI3Ka/ß/?, respectively. Torkinib (PP242) induces mitophagy and apoptosis.
nonionic liquid polymer of the alkyl aryl polyether alcohol type
 Adiponectin (mouse), (recombinant)
ALX522–059; Enzo Life Sciences. Mimics serum adiponectin by forming high molecular weight (HMW) and hexameric species. Alternative names-Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28.
Perfluorooctanoic acid 95% pure. Perfluorooctanoic acid is a perfluoroalkyl acid commonly used in the preparation of fluoroacrylic esters, fluoropolymers and fluoroelastmers. It is found to be environmentally persistent and bioaccumulative with a long hal
2,4-Dinitrophenol (DNP) is a precursor to other chemicals and is biochemically active, inhibiting adenosine triphosphate (ATP) production in cells with mitochondria.
This is a soluble epoxide hydrolase (sEH) inhibitor: 4-[[trans-4-[[(tricyclo[,7]dec-1-ylamino)carbonyl]amino]cyclohexyl]oxy]-benzoic acid in 1% v/v PEG400
BAM15: N5,N6-bis(2-fluorophenyl)-[1,2,5]oxadiazolo[3,4-b]pyrazine-5,6-diamine, a novel mitochondrial protonophore uncoupler capable of protecting mammals from acute renal ischemic-reperfusion injury and cold-induced microtubule damage
Niclosamide: inhibits glucose uptake and oxidative phosphorylation
 EDC Mixture
The concentrations of the EDCs are atrazine (10 mg/kg), bisphenol-A (50 µg/kg), perfluorooctanoic acid (0.1 mg/kg), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (0.036 µg/kg)
The compound is proprietary to Energesis Pharmaceuticals.
Doxycycline is a broad-spectrum tetracycline-class antibiotic used in the treatment of infection.
6'-Sialyllactose belongs to the class of organic compounds known as n-acylneuraminic acids. These are neuraminic acids carrying an N-acyl substituent. 6'-Sialyllactose is an extremely weak basic (essentially neutral) compound (based on its pKa). Sialyllac
Lacto-N-tetraose is a complex sugar found in human milk. It is one of the few characterized human milk oligosaccharides (HMOs) and is enzymatically synthesized from the substrate lactose. It is biologically relevant in the early development of the infant
3,6-dichlorobenzo[b]thiophene-2-carboxylic acid
 ERAP1 Inhibitor
Inhibitor of Endoplasmic-reticulum associated aminopeptidase 1 (Maben et al Biochemistry and Molecular Biology 2018).
Trulicity (dulaglutide) is a glucagon-like peptide-1 (GLP-1) receptor agonist. It works by activating the GLP-1 receptor
 Sucrose + Fructose
sucrose + fructose (Acros Organic, New Jersey, NY)
 Rebaudioside A
Rebaudioside A is a steviol glycoside from the leaves of Stevia rebaudiana that is 240 times sweeter than sugar. Rebaudioside A is the sweetest and most stable steviol glycoside, and is less bitter than stevioside.
Sucralose is an artificial sweetener and sugar substitute.
Semaglutide acts like human glucagon-like peptide-1 such that it increases insulin secretion, thereby increasing sugar metabolism.
 Semaglutide + EP CMPD
Semaglutide that was given in conjunction with the Energesis proprietary compound.
Naturally occurring human protein. Klotho is an enzyme that in humans is encoded by the KL gene. There are three subfamilies of klotho: a-klotho, ß-klotho, and ?-klotho. a-klotho activates FGF23, and ß-klotho activates FGF19 and FGF21. When the subfamily
Empagliflozin is a SGLT2 class drug used in the treatment of type 2 diabetes
Dihydrotestosterone is an endogenous androgen sex steroid and hormone, and agonist of the androgen receptor
Anti-inflammatory dietary supplement

Experimental Factor: Experimental Group

This animal belongs to experimental group that is homozygous for gene manipulations.
This animal belongs to the control group for the experiment.
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.
This animal belongs to the control group and is heterozygous wild type/mutation for the affected locus.
Experimental Factor (Units)
Drug Administered
Experimental Group

Phenotype Assays Add / Edit

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