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Experiment

IRS4cre+TetTox
Summary Data Summary
Investigator Olson, David
Description Assessing significance of PVH subpopulation of neurons in energy homeostasis in
rodents. Could be informative in human neurophysiology.
Status Completed
Public Release 3/21/2021
Animal Age Measured In: week(s) post-natal (w)
Flags
Data Analysis
TypeCount
Animals0
Experimental Conditions2
Catalog Items1
Curation Info (# flags)1
Phenotype Assays4
Phenotype Measurements0
Histology Images0
Publications0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
 Control
This animal belongs to the control group for the experiment.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.
 Control-Het
This animal belongs to the control group and is heterozygous wild type/mutation for the affected locus.

Experimental Factor: Virus Injected

 lacZ Adenovirus
Adenovirus with lacZ cassette
 Lpcat3 shRNA Adenovirus
Adenovirus that expresses an shRNA for lysophosphatidylcholine acyltransferase 3 (Lpcat3)
 Ad-shNOX4
Adenovirus that expresses an shRNA for NOX4
 Ad-shGFP
Adenovirus that expresses an shRNA for GFP
 Vehicle
Injection of vehicle without virus. Negative control.
 AAV-GFP
Adeno associated virus with over expressing green fluorescent protein (GFP)
 AAV-C5
Adeno associated virus with over expression of Human Complement C5
 AAV-B186-Vegfb
Adeno Associated Virus B186 over expresses Vegfb.
 AAV-Control
Empty Adeno Associate Virus control.
 Adenovirus IRE1a K599A
An Adenovirus expressing the K599A mutant of the IRE1a gene.
 Adenovirus 3xFLAG Sirt6
Adenoviruses were produced with the pAd-Track/pAd-Easy system. 3xFLAG Sirt6 adenoviruses were subcloned from pCMV7.1-3xFLAG constructs. Adenoviruses containing shRNAs were subcloned from pLKO.1 vectors with the U6 promoter into the...
 AAV-March1
Adeno associated virus with over expression of March1
 AAV8-Cre-GFP
AAV serotype 8 virus expresses Cre Recombinase and eGFP marker
 AAV8-Cre
AAV serotype 8 virus expresses Cre Recombinase
 AAV8-Lrh1
AAV serotype 8 virus expresses Lrh1
 AAV-PCSK9
Adeno associated virus with over expressing proprotein convertase subtilisin/kexin type 9
 AAV-DIO-mcherry
Control AAV virus is cre dependent for the expression of a fluorophore, indicating the cre positive neurons are infected by the virus.
 AAV-DIO-GFP:TetTox
AAV-TetTox is to cre-dependently express not only a fluorophore but also a protein called TetTox, which will block the virus infected cre positive neurons from receiving neurotransmitters/hormones/peptides from connected neurons.
 AAV-pCAG-Atg5
AAV9 encoding an over expression cassette for autophagy related 5 gene (Atg5).
 AAV-pCAG-Cisd2
AAV9 encoding an over expression cassette for CDGSH iron sulfur domain 2 (Cisd2).
 AAV-pCB7-mAFP
AAV9 encoding an over expression cassette for Mouse alphafetoprotein (mAFP).
 Coxsackie B4
Coxsackie B4 has a cell tropism for natural killer cells and pancreatic islet cells
 None
No virus was injected.
 MCMV
Mouse cytomegalovirus
 AAV8-miR375
miR375 treatment mediated via virus
 AAV8-CBG
AAV serotype 8 virus expresses corticosteroid-binding globulin (SERPINA6)
 AAV8
AAV serotype 8 virus
 AAV Klotho
An adeno-associated virus (AAV) carrying mouse klotho full-length cDNA.
 mCherry
Member of the mFruits family of monomeric red fluorescent proteins (mRFPs)
 Alk-mCherry
Expresses mCherry-ALK1 in mammalian cells.
 AAV8-Flex-Syn-EGFP
Synapsin promoter
 rAAV2-CMV-Alk_sgRNA
Alk-AAV serotype 2 virus
 AAV8-Syn-DIO-mCherry
AAV8-hSyn-DIO-mCherry is a pre-made rAAV in serotype 8 which will Cre dependently over-express mCherry driven under human synapsin promoter.
 AAV-CAG-Slug
Slug adenoviral vectors. Slug is a transcriptional regulator that contains an N-terminal SNAG domain and a C-terminal DNA-binding domain.
 AAV-CAG-GFP
AAV serotype virus expresses eGFP (Green Fluorescent Protein) under the control of CAG promoter. The CAG promoter is a hybrid of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter. The CAG promoter is used to drive high leve
 AAV-S6K2-FLAG
Cre-dependent adeno-associated virus serotype 8 (AAV8) vectors, cloned with p54-S6K2 protein fused with the FLAG peptide DNA sequence.
 AAV-Flex-hM3Dq
A modified human muscarinic receptor coupled to the stimulatory Gq-protein which activates neurons upon binding the synthetic ligand clozapine N-oxide. Cre-dependent AAV-Flex-hM3Dq-mCherry (AAV-Flex-hM3Dq, titer: 4 × 1012 virus molecules/mL, purchased fro
 AAV-Flex-TetTox
Tetanus toxin cleaves synaptobrevin, a component of the SNARE core and prevents release of synaptic vesicles from targeted neurons.
Experimental Factor (Units)
Experimental Group
Virus Injected


No documents found.

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