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Effect of 2-o-fucosyllactose on cognition with control or high fat diet

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Investigator	Raybould, Helen
Description	Milk oligosaccharides, including 2-O-fucosyllactose (2-FL) are naturally evolved prebiotic substrates that facilitate bifidobacterial enrichment and interaction with the host. We have evidence to show that prebiotic milk oligosaccharides will reduce gut and adipose tissue inflammation and reduce body weight gain in mice on high fat, high energy diets. Male mice ~6 weeks of age are place on control (RD D12450J) or high fat diet (RD D12451) +/- 2-O-fucosyllactose (10% bw in diet) for 8 weeks. Cognition was tested by y maze and novel object test. for the y maze, mice were adapted to testing room for 30 min, were placed in the center of the Y-maze and were tracked with an overhead camera for the extent of a 6-min trial. An elevated white plastic Y-maze with three 35 cm arms at 120-degree angles. For novel object, day 1 consisted of a 15 min environmental habituation which also served as our open field test. Day 2 consisted of a 12 min familiarization period with two identical objects (plastic flasks or cones) and a 12 min test with one known and one novel object (one flask and one cone). Mice, adapted to testing room for 60 min, were placed in the center of a white plastic box (40 x 40x 40 cm) and were tracked with an overhead camera. The familiarization period and novel object test were performed 4 hr. apart. The time spent at both the novel and known objects were recorded. The percentage of time exploring the novel object (% novel) was determined when the total exploration time was 20 sec. Applicable research areas: Metabolism, Neurobiology, and Obesity
Status	Completed
Public Release	4/14/2022
Animal Age	Measured In: week(s) post-natal (w)
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Type	Count
Animals	32
Experimental Conditions	6
Catalog Items	1
Curation Info (# flags)	1
Phenotype Assays	2
Phenotype Measurements	64
Histology Images	0
Publications	0

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	32	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	2-Fucosyllactose	2'-Fucosyllactose is an oligosaccharide. It is the most prevalent human milk oligosaccharide (HMO), making up about 30% of all of HMOs.
	Experimental Factor: Drug Delivery Route
	Diet	The drug was delivered via food.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	Research Diets - D12450J	Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
	Research Diets - D12451	Research Diets - D12451 Rodent Diet with 45% kcal% fat

Experimental Factor (Units)
Drug Administered
Drug Delivery Route
Drug Dose (AU)
Drug Dose Units
Experimental Group
Mouse Diet

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
alternating triplets - percent	% triplets
Novel exploration-percent	Novel exploration-pe

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