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Experiment

Hyperinsulinemic Euglycemic Clamp Order #1667 6/10/09
Summary Data Summary
Investigator Sakamoto, Kei
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT and KO

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.
Status Completed
Public Release 3/31/2012
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainMetadataExperiment Description
Data Analysis
TypeCount
Animals17
Experimental Conditions3
Catalog Items1
Curation Info (# flags)3
Phenotype Assays20
Phenotype Measurements1086
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
7
0
TBCD14 KO
0
10
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Insulin Infusion Rate - milliUnits (mU/kg/min)

 Insulin Infusion Rat
This is insulin infusion rate for hyperinsulinemic clamp data

Experimental Factor: Time Sample Taken (min)

 Time Sample Taken
Time in minutes since a sample was taken


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