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Experiment

Experiment for Order #1795
Summary Data Summary
Investigator Huen, Sarah
Description BRP-39 has been shown to be involved in regulating the innate immune response
after lung models of oxidative injury and hypersensitivity reactions. We are
hypothesizing that it is also involved in the innate immune response after
ischemia reperfusion injury in the kidney.

Mouse breast regression protein 39 (BRP-39; Chi3l1) and its human homologue
YKL-40 are chitinase-like proteins that lack chitinase activity. Although YKL-40
is expressed in exaggerated quantities and correlates with disease activity in
asthma and many other disorders, the biological properties of BRP-39/YKL-40 have
only been rudimentarily defined. We describe the generation and characterization
of BRP-39?/? mice, YKL-40 transgenic mice, and mice that lack BRP-39 and produce
YKL-40 only in their pulmonary epithelium. Studies of these mice demonstrated
that BRP-39?/? animals have markedly diminished antigeninduced Th2 responses and
that epithelial YKL-40 rescues the Th2 responses in these animals. The ability
of interleukin13 to induce tissue inflammation and fibrosis was also markedly
diminished in the absence of BRP-39. Mechanistic investigations demonstrated
that BRP-39 and YKL-40 play an essential role in antigen sensitization and
immunoglobulin E induction, stimulate dendritic cell accumulation and
activation, and induce alternative macrophage activation. These proteins also
inhibit inflammatory cell apoptosis/cell death while inhibiting Fas expression,
activating protein kinase B/AKT, and inducing Faim 3. These studies establish
novel regulatory roles for BRP-39/YKL-40 in the initiation and effector phases
of Th2 inflammation and remodeling and suggest that these proteins are
therapeutic targets in Th2- and macrophage-mediated disorders.
Status Completed
Public Release 5/20/2012
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainMetadataExperiment Description
Data Analysis
TypeCount
Animals14
Experimental Conditions1
Catalog Items1
Curation Info (# flags)3
Phenotype Assays1
Phenotype Measurements14
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
8
0
C57BL/6-Brp39-/-
0
6
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


Phenotype Assays Add / Edit


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