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Experiment

Experiment for Order #1846
Summary Data Summary
Investigator Nasiri, Ali
Description To gain insight into understanding the cellular changes that may occur in
articular cartilage, we conducted experiments in Hyp mice, a murine model that
genocopies and phenocopies human XLH and one that we have previously used to
explore and characterize the enthesopathy of XLH. Specifically, we performed
equilibrium partitioning of an ionic contrast (EPIC)-microCT analysis to obtain
quantitative, high-resolution, three-dimensional images of articular cartilage
of 7-month-old control and Hyp mice to determine if articular cartilage thinning
is characteristic of Hyp mice. We also examined articular cartilage at skeletal
maturity to determine if Hyp mice display intrinsic cartilage abnormalities that
might predispose them to degenerative osteoarthropathy and compared these
findings to Hyp mice undergoing conventional treatment for XLH during long bone
growth. Our findings provide a rationale for both early and long-term treatment
in order to preserve both the anatomical and biochemical characteristics that
define the unique articular cartilage architecture that has evolved to
accommodate mechanical loads.

Applicable research area(s): Endocrine deficiency, Metabolism, Developmental
Biology
Status Completed
Public Release 6/2/2012
Data Collected? Data will not be collected for this catalog item
Animal Age Measured In: month(s) post-natal (m)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals15
Experimental Conditions1
Catalog Items2
Curation Info (# flags)2
Phenotype Assays2
Phenotype Measurements30
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
3
4
0
PhexHyp-2J
6
2
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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