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Experiment

Liver and muscle lipid levels in Myc KO mice fed high fat and high cholesterol
diet
Summary Data Summary
Investigator Rodrigues, Claudia
Description For the FVB/N-Tg(tetO-MYC)36aBop/J animals, doxycycline was withdrawn at 4 weeks
and the experimental diet started at 8 weeks of age. Then the animals were
fasted for 6 hours and anesthetized intraperitoneally with a ketamine-xylazine
solution. Blood was collected by cardiac puncture in EDTA-coated tubes. Liver
and muscle was harvested, snap frozen with liquid N2 and then stored at -80
degree.
Status Completed
Public Release 3/26/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagAnimal CountNo dataMouse Diet
Data Analysis
TypeCount
Animals15
Experimental Conditions5
Catalog Items6
Curation Info (# flags)3
Phenotype Assays2
Phenotype Measurements48
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
FVB/N +/+
0
6
0
FVB/N-Tg(tetO-MYC)36aBop/J
0
9
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 Teklad - 02028
Modification of TD.88137, to increase added cholesterol to 1.25% (total in the diet is 1.3%) and add 0.5% cholic acid, 15.4% kcal Protein, 41.9% kcal Carb, 42.6% kcal Fat
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)

Experimental Factor: Tissue Source

 liver
liver
 muscle
muscle
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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