MMPC :: Experiment

Experiment

Effect of 2-o-fucosyllactose on open field performance with control or high fat
diet

Summary

Data Summary

Investigator	Raybould, Helen
Description	Milk oligosaccharides, including 2-O-fucosyllactose (2-FL) are naturally evolved prebiotic substrates that facilitate bifidobacterial enrichment and interaction with the host. We have evidence to show that prebiotic milk oligosaccharides will reduce gut and adipose tissue inflammation and reduce body weight gain in mice on high fat, high energy diets. Male mice ~6 weeks of age are place on control (RD D12450J) or high fat diet (RD D12451) +/- 2-O-fucosyllactose (10% bw in diet) for 8 weeks. Mice, adapted to testing room for 60 min, were placed in the center of a white plastic box (40 x 40x 40 cm) and were tracked with an overhead camera for 15 min. Distance traveled and time spent in the perimeter (outside 10 cm) vs center (inside 30 cm) was determined. Applicable research areas: Metabolism, Neurobiology, and Obesity
Status	Completed
Public Release	4/15/2022
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	32	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	2-Fucosyllactose	2'-Fucosyllactose is an oligosaccharide. It is the most prevalent human milk oligosaccharide (HMO), making up about 30% of all of HMOs.
	Experimental Factor: Drug Delivery Route
	Diet	The drug was delivered via food.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	Research Diets - D12450J	Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
	Research Diets - D12451	Research Diets - D12451 Rodent Diet with 45% kcal% fat
	Experimental Factor: Open Field Zone
	Perimeter	Animal was in the perimeter of the open field in the arena.

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
Time in Open Field Zone	Time in OPZ
Distance in Open Field Zone	Distance in OFZ

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