MMPC :: Experiment

Experiment

Effect of 6'-Sialyllactose on open field performance with control or high fat
diet

Summary

Data Summary

Investigator	Raybould, Helen
Description	To determine if addition of 6'-Sialyllactose ameliorates the cognitive impairment effects of a high fat diet. Mice will be fed either a low fat control (D12450J, equivalent) or high fat (D12451, equivalent) and provided 6'SL by drinking water (10%) for 8 weeks. All 32 mice will then undergo open field test. For this, mice are adapted to testing room for 60 min, were placed in the center of a white plastic box (40 x 40x 40 cm) and were tracked with an overhead camera for 15 min. Distance traveled and time spent in the perimeter (outside 10 cm) vs center (inside 30 cm) was determined.
Status	Completed
Public Release	2/9/2024
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	31	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	6-Sialyllactose	6'-Sialyllactose belongs to the class of organic compounds known as n-acylneuraminic acids. These are neuraminic acids carrying an N-acyl substituent. 6'-Sialyllactose is an extremely weak basic (essentially neutral) compound (based on its pKa). Sialyllac
	Experimental Factor: Drug Delivery Route
	Drinking Water	The drug was delivered in the drinking water.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	Research Diet - D12450J	Rodent Diet with 10% kcal% fat. The fat comes from Lard and Soybean Oil
	Research Diets - D12451	Research Diets - D12451 Rodent Diet with 45% kcal% fat
	Experimental Factor: Open Field Zone
	Center	Animal was in the center of the open field zone of the arena.
	Perimeter	Animal was in the perimeter of the open field in the arena.

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
Time in Open Field Zone	Time in OPZ
Distance in Open Field Zone	Distance in OFZ

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