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Experiment

Hyperglycemic clamp order#25877 2019
Summary Data Summary
Investigator Collins, Samantha
Description Immunodeficient mouse model - used in tumor biology and xenograft research.
Mice 1 – 9: Transplanted with 5 million embryonic stem-cell (ESC) - derived beta
cells (SC-betas) under the renal capsule on January 9, 2019. These cells were
differentiated from the pathogen free HUES8 cell line in our lab with use of our
differentiation protocol. SC-betas have been shown to have the capacity to
develop into teratomas in vivo, and as such are monitored weekly for tumor
production. Attached below is the protocol we have submitted to IACUC at Harvard
for tumor monitoring. The tumor monitoring has been communicated with the
quarantine team at Vanderbilt.
Mice 10-19 were each transplanted under the renal capsule with 500 IEQ of
cadaveric human islets supplied to use by Prodo Laboratories. There are no
concerns for human pathogens for these tissues.
Status Completed
Public Release 3/5/2023
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountNo dataExperiment DescriptionNeed Conditions
Data Analysis
TypeCount
Animals10
Experimental Conditions4
Catalog Items9
Curation Info (# flags)5
Phenotype Assays6
Phenotype Measurements603
Histology Images0
Publications0
Phenotypes1


Animals
Strain NameCommon NameFemalesMalesUnknown
B6.CB17-Prkdc(scid)Lyst(bg-J)/Crl
0
10
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...

Experimental Factor: Treatment Type

 Beta cells
Transplanted with 5 million embryonic stem-cell (ESC) - derived beta cells (SC-betas) under the renal capsule.
 Human islets
Transplanted under the renal capsule with 500 IEQ of cadaveric human islets supplied to use by Prodo Laboratories. There are no concerns for human pathogens for these tissues.
Experimental Factor Values


No documents found.

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