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Sable Energy Balance and Max Test 12/29/14 #18483
Summary Data Summary
Investigator Wasserman, David
Description Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 5 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Then energy expenditure measured by indirect calorimetry in the Oxymax by
Columbus Instruments. Mice were trained to run on the treadmill by running them
on a treadmill for 10 minutes at 10 m/min 2 to 3 days prior to the experiment.
On the day of the experiment mice were placed in the treadmill attached to the
Oxymax. The treadmill was turned off for 30 minutes to take readings of the
animal rested. Mice were then run on a treadmill at gradually increasing speeds
until exhaustion. When the mice became exhausted the treadmill was turned off
and recordings were taken for another 30 minutes. Speeds were increased by 4
m/min every 3 minutes, with the starting speed set to 10 m/min.

Sable run 1 is the Sable run, Sable run 2 is the treadmill run.

Male WT (C56BL/6J <+/+> ) and SIRT3 KO (C57BL/6J-Sirt3
[C57BL/6J-Sirt3<-/->]) mice.
Mice were fed a 60% kcal High Fat Diet (F3282 BioServ).

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure, feeding behaviors, and different
exercise capacity.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
Status Completed
Public Release 4/8/2017
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagExperiment Description
Data Analysis
Experimental Conditions4
Catalog Items1
Curation Info (# flags)1
Phenotype Assays15
Phenotype Measurements384
Histology Images0

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 BioServ F3282
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix

Experimental Factor: Sable Run (RNum)

 Sable Run
The run number for the Sable system used to assess energy expenditure.

Experimental Factor: Treadmill Speed (m/min)

The speed of the treadmill used during an energy expenditure experiment

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