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Experiment

Hyperinsulinemic Euglycemic Clamp Order #12021,October 2014
Summary Data Summary
Investigator Kolumam, Ganesh
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.

Groups:
Male WTand 129
Mice were given HAb or HAb-FGFR1
Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance
Status Completed
Public Release 6/3/2017
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals37
Experimental Conditions5
Catalog Items2
Curation Info (# flags)2
Phenotype Assays18
Phenotype Measurements10279
Histology Images0
Publications0
Phenotypes7


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
18
0
B6;129-Adipoq-/-/J
0
19
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 HAb-Control
Control humanized antibody
 HAb-FGFR1
Humanized bispecific antibody that selectively activates FGFR1/ß Klotho complex

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor Values


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