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Experiment

Liver lipids in db/db mice treated with Methionine and Choline Deficient (MCD)
Diet or MCD plus niclosamide for 3 weeks

Summary

Data Summary

Investigator	Shengkan, Jin
Description	10 week old female B6.BKS(D)-Leprdb /J mice were treated with Methionine and Choline Deficient (MCD) Diet or MCD plus niclosamide for 3 weeks. After 5 hour fasting, mice were anesthetized with ketamine and xylazine first for blood draw from heart, followed by liver tissue collection. Liver tissue were then kept in liquid nigtrogen for further analysis. Applicable research area(s): Diabetes, Metabolism
Status	Completed
Public Release	7/22/2017
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

DOWNLOAD ALL DATA (.csv)

Type	Count
Animals	16
Experimental Conditions	5
Catalog Items	3
Curation Info (# flags)	1
Phenotype Assays	2
Phenotype Measurements	32
Histology Images	0
Publications	0

Animals

Strain Name	Common Name	Females	Males	Unknown
B6.BKS(D)-Lepr^db/J	B6.BKS(D)-Lepr^db/J	16	0	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Experimental Factor: Mouse Diet
	Research Diets - A02082002B	Methionine/choline deficient diet with 60 kcal% fat for NASH Models
	Research Diets - A02082002B 2000 ppm NEN	Methionine/choline deficient diet with 60 kcal% fat for NASH Models supplemented with 2000 ppm niclosamide ethanolamine (NEN)
	Experimental Factor: Tissue Source
	liver	liver

Experimental Factor (Units)
Experimental Group
Fast Duration (hr)
Feeding Group
Mouse Diet
Tissue Source

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
triglycerides, tissue	TG (tissue)
free fatty acids, tissues	FFA tissue

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