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Publication
A novel, noninvasive method for the measurement of intestinal fat absorption.
Authors Jandacek RJ, Heubi JE, Tso P
Submitted By Patrick Tso on 11/6/2007
Status Published
Journal Gastroenterology
Year 2004
Date Published 7/1/2004
Volume : Pages 127 : 139 - 144
PubMed Reference 15236180
Abstract BACKGROUND & AIMS: The goal of the study was to facilitate fat balance
measurements with an appropriate intestinal marker for the transit of dietary
fat and thereby eliminate the need for complete diet and fecal collections.
METHODS: Dietary fat containing 5% sucrose polybehenate was fed in a
semisynthetic diet to rats and mice. Fat absorption was calculated from the
ratios of behenic acid to other fatty acids in diet and feces as analyzed by gas
chromatography of fatty acid methyl esters. The method was validated by
measuring absorption of well-absorbed (safflower oil) and poorly absorbed
(olestra; calcium soaps) dietary fats. The animals were fed meals containing
test fats for 2 or 3 days, and fecal samples were collected. Fecal samples of
approximately 10 mg (single fecal pellet from mice) were assayed. RESULTS: The
method yielded values that were consistent with complete absorption of safflower
oil and the nonabsorbability of olestra and calcium soaps. The results were
reproducible and consistent among individual fecal aliquots. The method was
compared with traditional fat-balance methods in animals fed both high- and
low-fat diets. CONCLUSIONS: Sucrose polybehenate is an appropriate marker that
allows the rapid measurement of fat absorption by analyzing aliquots of <1% of
total feces. The method is noninvasive, does not require isotope analyses, and
can be carried out as part of an animal's normal feeding regimen. The method may
be a facile technique to assess fat absorption measurements in humans.




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