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Publication
Temporal changes in ventricular function assessed echocardiographically in
conscious and anesthetized mice.
Authors Rottman JN, Ni G, Khoo M, Wang Z, Zhang W, Anderson ME, Madu EC
Submitted By Jeff Rottman on 11/12/2007
Status Published
Journal Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography
Year 2003
Date Published 11/1/2003
Volume : Pages 16 : 1150 - 1157
PubMed Reference 14608286
Abstract The mouse is an important model system for cardiovascular biology, with
echocardiography a critical tool for noninvasive measurement of cardiac
morphology and function. The feasibility and short-term temporal consistency of
repeated echocardiographic measurements in conscious mice has not been
previously evaluated. We performed serial 2-dimensional guided M-mode
transthoracic echocardiographic measurements at 5- to 10-minute intervals over
60 minutes in conscious mice and in mice treated with 1 of 3 anesthetic
regimens: ketamine and acepromazine (n = 14); pentobarbital (n = 14); and
ketamine and xylazine (n = 13). Unanesthetized mice received intraperitoneal
saline (n = 6) or no injection (n = 7). In sequentially repeated measurements
over 1 hour in conscious mice, none of the measured or derived echocardiographic
parameters differed from baseline, whereas all 3 anesthetic regimens produced
significant, prolonged, and temporally variable decreases in heart rate and
fractional shortening. The relationship between heart rate and fractional
shortening was not altered by anesthetic choice. Serial echocardiographic
assessments of cardiac function, dimension, and mass can be performed with high
reproducibility in conscious mice.




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