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Publication
Glucose metabolism in vivo in four commonly used inbred mouse strains.
Authors Berglund ED, Li CY, Poffenberger G, Ayala JE, Fueger PT, Willis SE, Jewell MM,
Powers AC, Wasserman DH
Submitted By David Wasserman on 7/3/2008
Status Published
Journal Diabetes
Year 2008
Date Published 7/1/2008
Volume : Pages 57 : 1790 - 1799
PubMed Reference 18398139
Abstract OBJECTIVE: To characterize differences in whole-body glucose metabolism between
commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS:
Hyperinsulinemic-euglycemic (approximately 8.5 mmol/l) and -hypoglycemic
(approximately 3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to
assess insulin action and hypoglycemic counter-regulatory responsiveness.
Hyperglycemic clamps (approximately 15 mmol/l) were done to assess insulin
secretion and compared with results in perifused islets. RESULTS: Insulin action
and hypoglycemic counter-regulatory and insulin secretory phenotypes varied
considerably in four inbred mouse strains. In vivo insulin secretion was
greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia
was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin
secretion, relative hepatic insulin resistance, and the highest
counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was
lowest among the strains, and islets isolated had the greatest
glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo
physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were
intermediate relative to other strains. Insulin secretion by C57BL/6 mice was
similar to that in other strains in contrast to the blunted glucose-stimulated
insulin secretion from isolated islets. CONCLUSIONS: Strain-dependent
differences exist in four inbred mouse strains frequently used for genetic
manipulation and study of glucose metabolism. These results are important for
selecting inbred mice to study glucose metabolism and for interpreting and
designing experiments.




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