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Publication
Markers of glycemic control in the mouse: comparisons of 6-h- and
overnight-fasted blood glucoses to Hb A1c.
Authors Han BG, Hao CM, Tchekneva EE, Wang YY, Lee CA, Ebrahim B, Harris RC, Kern TS,
Wasserman DH, Breyer MD, Qi Z
Submitted By Raymond Harris on 2/12/2009
Status Published
Journal American journal of physiology. Endocrinology and metabolism
Year 2008
Date Published 10/1/2008
Volume : Pages 295(4) : E981 - E986
PubMed Reference 18664598
Abstract The present studies examined the relationship between fasting blood glucose and
Hb A(1c) in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes
mellitus. Daily averaged blood glucose levels based on continuous glucose
monitoring and effects of 6-h vs. overnight fasting on blood glucose were
determined. Daily averaged blood glucose levels were highly correlated with Hb
A(1c), as determined with a hand-held automated device using an immunodetection
method. R(2) values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J,
respectively. Six-hour fasting blood glucose correlated more closely with the
level of daily averaged blood glucose and with Hb A(1c) than did blood glucose
following an overnight fast. To validate the immunoassay-determined Hb A(1c), we
also measured total glycosylated hemoglobin using boronate HPLC. Hb A(1c) values
correlated well with total glycosylated hemoglobin in all three strains but were
relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice.
These results show that 6-h fasting glucose provides a superior index of
glycemic control and correlates more closely with Hb A(1c) than overnight-fasted
blood glucose in these strains of mice.




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