mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
Long chain fatty acid uptake in vivo: comparison of [125I]-BMIPP and
[3H]-bromopalmitate.
Authors Shearer J, Coenen KR, Pencek RR, Swift LL, Wasserman DH, Rottman JN
Submitted By Larry Swift on 2/12/2009
Status Published
Journal Lipids
Year 2008
Date Published 8/1/2008
Volume : Pages 43(8) : 703 - 711
PubMed Reference 18481132
Abstract Insulin resistance is characterized by increased metabolic uptake of fatty
acids. Accordingly, techniques to examine in vivo shifts in fatty acid
metabolism are of value in both clinical and experimental settings. Partially
metabolizable long chain fatty acid (LCFA) tracers have been recently developed
and employed for this purpose: [9,10-3H]-(R)-2-bromopalmitate ([3H]-BROMO) and
[125I]-15-(rho-iodophenyl)-3-R,S-methylpentadecanoic acid ([125I]-BMIPP). These
analogues are taken up like native fatty acids, but once inside the cell do not
directly enter beta-oxidation. Rather, they become trapped in the slower
processes of omega and alpha-oxidation. Study aims were to (1) simultaneously
assess and compare [3H]-BROMO and [125I]-BMIPP and (2) determine if tracer
breakdown is affected by elevated metabolic demands. Catheters were implanted in
a carotid artery and jugular vein of Sprague-Dawley rats. Following 5 days
recovery, fasted animals (5 h) underwent a rest (n = 8) or exercise (n = 8) (0.6
mi/h) protocol. An instantaneous bolus containing both [3H]-BROMO and
[125I]-BMIPP was administered to determine LCFA uptake. No significant
difference between [125I]-BMIPP and [3H]-BROMO uptake was found in cardiac or
skeletal muscle during rest or exercise. In liver, rates of uptake were more
than doubled with [3H]-BROMO compared to [125I]-BMIPP. Analysis of tracer
conversion by TLC demonstrated no difference at rest. Exercise resulted in
greater metabolism and excretion of tracers with approximately 37% and
approximately 53% of [125I]-BMIPP and [3H]-BROMO present in conversion products
at 40 min. In conclusion, [3H]-BROMO and [125I]-BMIPP are indistinguishable for
the determination of tissue kinetics at rest in skeletal and cardiac muscle.
Exercise preferentially exacerbates the breakdown of [3H]-BROMO, making
[125I]-BMIPP the analogue of choice for prolonged (>30 min) experimental
protocols with elevated metabolic demands.




Catalog Groups / Subjects

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.