mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
The intestinal lymph fistula model--a novel approach to study ghrelin secretion.
Authors Tong J, Tschöp MH, Aulinger BA, Davis HW, Yang Q, Liu J, Gaylinn BD, Thorner MO,
D'Alessio D, Tso P
Submitted By Matthias Tschop on 3/10/2010
Status Published
Journal American journal of physiology. Gastrointestinal and liver physiology
Year 2010
Date Published 3/1/2010
Volume : Pages 298 : Not Specified
PubMed Reference 20044509
Abstract The orexigenic hormone ghrelin is secreted from the stomach and has been
implicated in the regulation of energy and glucose homeostasis. We hypothesized
that ghrelin, like other gastrointestinal (GI) hormones, is present in
intestinal lymph, and sampling this compartment would provide advantages for
studying ghrelin secretion in rodents. Blood and lymph were sampled from
catheters in the jugular vein and mesenteric lymph duct before and after
intraduodenal (ID) administration of isocaloric Ensure, dextrin, or Liposyn
meals or an equal volume of saline in conscious Sprague-Dawley rats. Total
ghrelin levels were measured using an established radioimmunoassay. Acyl and
des-acyl ghrelin were measured using two-site ELISA. Fasting ghrelin levels in
lymph were significantly higher than in plasma (means +/- SE: 3,307.9 +/- 272.9
vs. 2,127.1 +/- 115.0 pg/ml, P = 0.004). Postingestive acyl and des-acyl ghrelin
levels were also significantly higher, whereas the ratio of acyl:des-acyl
ghrelin was similar in lymph and plasma (0.91 +/- 0.28 vs. 1.20 +/- 0.36, P =
0.76). The principle enzymes responsible for deacylation of ghrelin were lower
in lymph than in plasma. Following ID Ensure, maximum ghrelin suppression
occurred at 2 h in lymph compared with at 1 h in plasma. The return of
suppressed ghrelin levels to baseline was also delayed in lymph. Similarly,
dextrin also induced significant suppression of ghrelin (two-way ANOVA: P =
0.02), whereas Liposyn did not (P = 0.32). On the basis of these findings, it
appears that intestinal lymph, which includes drainage from the interstitium of
the GI mucosa, is enriched in ghrelin. Despite reduced deacylating activity in
lymph, there is not a disproportionate amount of acyl ghrelin in this pool. The
postprandial dynamics of ghrelin are slower in lymph than plasma, but the
magnitude of change is greater. Assessing ghrelin levels in the lymph may be
advantageous for studying its secretion and concentrations in the gastric
mucosa.




Catalog Groups / Subjects

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.