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Publication
The influence of shc proteins and aging on whole body energy expenditure and
substrate utilization in mice.
Authors Stern JH, Kim K, Ramsey JJ
Submitted By Submitted Externally on 11/3/2015
Status Published
Journal PLoS ONE
Year 2012
Date Published
Volume : Pages 7 : e48790
PubMed Reference 23144971
Abstract While it has been proposed that Shc family of adaptor proteins may influence
aging by regulating insulin signaling and energy metabolism, the overall impact
of Shc proteins on whole body energy metabolism has yet to be elucidated. Thus,
the purpose of this study was to determine the influence of Shc proteins and
aging on whole body energy metabolism in a mouse model under ambient conditions
(22°C) and acute cold exposure (12°C for 24 hours). Using indirect respiration
calorimetry, we investigated the impact of Shc proteins and aging on EE and
substrate utilization (RQ) in p66 Shc-/- (ShcKO) and wild-type (WT) mice.
Calorimetry measurements were completed in 3, 15, and 27 mo mice at 22°C and
12°C. At both temperatures and when analyzed across all age groups, ShcKO mice
demonstrated lower 24 h total EE values than that of WT mice when EE data was
expressed as either kJ per mouse, or adjusted by body weight or crude organ mass
(ORGAN) (P=0.01 for all). The ShcKO mice also had higher (P<0.05) fed state RQ
values than WT animals at 22°C, consistent with an increase in glucose
utilization. However, Shc proteins did not influence age-related changes in
energy expenditure or RQ. Age had a significant impact on EE at 22°C, regardless
of how EE data was expressed (P<0.05), demonstrating a pattern of increase in EE
from age 3 to 15 mo, followed by a decrease in EE at 27 mo. These results
indicate a decline in whole body EE with advanced age in mice, independent of
changes in body weight (BW) or fat free mass (FFM). The results of this study
indicate that both Shc proteins and aging should be considered as factors that
influence energy expenditure in mice.




Strains
StrainDevelopment StatusCreation MethodOptions
B6.Cg-Shc1tm1PgpPhenotyping ongoingknockout
View




Genes
SymbolDescription
Shc1src homology 2 domain-containing transforming protein C1

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