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Publication
Impact of murine intestinal apolipoprotein A-IV expression on regional lipid
absorption, gene expression, and growth.
Authors Simon T, Cook VR, Rao A, Weinberg RB
Submitted By Submitted Externally on 7/27/2017
Status Published
Journal Journal of lipid research
Year 2011
Date Published
Volume : Pages 52 : 1984 - 94
PubMed Reference 21840868
Abstract Apolipoprotein A-IV (apoA-IV) is synthesized by intestinal enterocytes during
lipid absorption and secreted into lymph on the surface of nascent chylomicrons.
A compelling body of evidence supports a central role of apoA-IV in facilitating
intestinal lipid absorption and in regulating satiety, yet a longstanding
conundrum is that no abnormalities in fat absorption, feeding behavior, or
weight gain were observed in chow-fed apoA-IV knockout (A4KO) mice. Herein we
reevaluated the impact of apoA-IV expression in C57BL6 and A4KO mice fed a
high-fat diet. Fat balance and lymph cannulation studies found no effect of
intestinal apoA-IV gene expression on the efficiency of fatty acid absorption,
but gut sac transport studies revealed that apoA-IV differentially modulates
lipid transport and the number and size of secreted triglyceride-rich
lipoproteins in different anatomic regions of the small bowel. ApoA-IV gene
deletion increased expression of other genes involved in chylomicron assembly,
impaired the ability of A4KO mice to gain weight and increase adipose tissue
mass, and increased the distal gut hormone response to a high-fat diet. Together
these findings suggest that apoA-IV may play a unique role in integrating
feeding behavior, intestinal lipid absorption, and energy storage.




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