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Strain
129S6/SvEvTac-Cpt1atm1Pwo

Summary Data Summary
Official Name 129S6/SvEvTac-Cpt1atm1Pwo
Common Name C57BL/6J-Cpt1a-/-
Description The Cpt-1a targeting vector was constructed from genomic DNA
fragments derived from a mouse 129X1/SvJ genomic P1 clone,
PV1. The P1 clone was identified by screening a mouse
129X1/SvJ strain genomic library by PCR (Genome Systems, St.
Louis, MO). Exons 11–18 were deleted by a replacement gene
targeting strategy by gene transfer into ES cells. The
targeted ES cells were used to generate mice with a null
allele (Cpt-1atm1Uab). ES cells (TC-1) were originally
derived from 129S6/SvEv mice. Screening for recombinant ES
cell clones was done by G418 selection (350 µg/ml) for 7
days. Surviving colonies were picked and expanded for
Southern blot analysis.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Philip WoodUniversity of Alabama at Birmingham


Genomic Information
GeneAllele 1Allele 2Protocol
Cpt1aknockoutknockoutNot Specified


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