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Strain
B6.129S-Gpamtm1Rcol/J

Summary Data Summary
Official Name B6.129S-Gpamtm1Rcol/J
Common Name B6.129S-Gpamtm1Rcol/J
Description A targeting vector containing neomycin resistance and herpes
simplex virus thymidine kinase genes was used to disrupt
0.5kb of sequence encoding 62 amino acids in homology
regions II and III. The construct was electroporated into
129S6/SvEvTac derived TC-1 embryonic stem (ES) cells.
Correctly targeted ES cells were injected into recipient
blastocysts. The resulting chimeric male animals were
crossed to C57BL/6J female mice, and then backcrossed to the
same for 6 generations.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
Phenotype Description Mice that are homozygous for the targeted mutation are
viable, fertile, and do not display any gross behavioral
abnormalities. No gene product (mRNA) is detected by
Northern blot analysis. Enzyme activity levels in liver
tissue are negligible. Residual activity is due to the
inactivated microsomal isoform. Homozygotes exhibit reduced
body weight. Female homozygotes weigh 20% less than wildtype
controls at age 10 months. Male homozygotes do not exhibit
as significant a weight reduction. Gonadal fat pad mass is
reduced. Liver triacylglycerol and plasma lipid levels are
reduced by 37% and 15% respectively. Very low density
lipoprotein (VLDL) triacylglycerol level and secretion are
decreased. Hepatic triacylglycerol fatty acid and
phospholipid fatty acid compositions are abnormal with
diminished palmitate content. F2 mice on a 50% C57BL/6J and
50% 129SvEv genetic background were used in all of the
experiments described in the primary reference. This mutant
mouse strain may be useful in studies of fatty acid
metabolism, lipid homeostasis and triacylglycerol and
phospholipid synthesis regulation.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Nora KoryYale University


Genomic Information
GeneAllele 1Allele 2Protocol
GpamknockoutknockoutNot Specified


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