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A PGK-neomycin resistance cassette replaced all of the
Serpine1 coding sequence and part of the promoter region
including the transcription initiation site. Northern blot
analysis did not detect Serpine1 mRNA in endotoxin-treated
homozygous mutant liver. Neither untreated nor
endotoxin-treated homozygous mutant kidney produced
detectable SERPINE1 immunoreactivity. A monoclonal antibody
based immunofunctional assay did not detect protein levels
in plasma of endotoxin-treated homozygous mutant mice.
Genomics - Modifications
Curation Flag Information
New comment to be added:
Tail Cuff Blood Pressure Order #181 3/16/03
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