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Strain
NOD.CB17-Prkdcscid/J

Summary Data Summary
Official Name NOD.CB17-Prkdcscid/J
Common Name NOD.CB17-Prkdcscid/J
Description A T-to-A transversion point mutation at a position
corresponding to codon 4095 created a premature stop codon.

Prkdc scid occurred spontaneously in a colony of BALB/c-Ighb
(C.B-17) mice maintained at the Institute for Cancer
Research in Philadelphia, PA. The Prkdcscid mutation was
backcrossed onto the NOD/ShiLt background as follows: an
NOD/ShiLt female was bred to a C.B-17-Prkdcscid male; male
Prkdcscid/+ offspring of the F1/N1 and subsequent
generations were mated to NOD/ShiLt females for a total of
10 crosses to NOD/ShiLt; at generation N10, Prkdcscid was
made homozygous by brother-sister inbreeding.
Development Status Phenotyping final
Creation Method spontaneous
Background NOD/Shi
Breeding Type intercross
Phenotype Description Mice homozygous for the severe combined immune deficiency
spontaneous mutation (Prkdcscid, commonly referred to as
scid) are characterized by an absence of functional T cells
and B cells, lymphopenia, hypogammaglobulinemia, and a
normal hematopoietic microenvironment. Normal
antigen-presenting cell, myeloid, and NK cell functions are
strain dependent. scid mice carry a DNA repair defect and a
defect in the rearrangement of genes that code for
antigen-specific receptors on lymphocytes. Most homozygotes
have no detectable IgM, IgG1, IgG2a, IgG2b, IgG3, or IgA.
Thymus, lymph nodes, and splenic follicles are virtually
devoid of lymphocytes. scid mice accept allogeneic and
xenogeneic grafts making them an ideal model for cell
transfer experiments. Some scid mice will spontaneously
develop partial immune reactivity. scid mice that have serum
Ig levels greater than 1 ug/ml are considered "leaky." scid
leakiness is highly strain dependent, increases with age,
and is higher in mice housed under non-SPF conditions. In
general, scid leakiness is high on the C57BL/6J and BALB/cBy
genetic backgrounds, low on the C3H/HeJ background, and even
lower on the NOD/ShiLtSz background. NOD/ShiLtSz-Prkdcscid
mice are both insulitis- and diabetes-free throughout life
and serve as a diabetes-free control for comparison to
NOD/ShiLtJ mice (Stock No. 001976). Thymic lymphomas occur
with high frequency, however, and life span typically is
limited to only 8.5 months under specific pathogen-free
conditions. In addition to being an excellent host for
xenografts, NOD.CB17-Prkdcscid/J mice may be useful for
delineation of the role of T cell subsets in autoimmune
diabetes and also as a source for insulitis-free islets.
TypeCount
Genomics - Modifications 1
Experiments 1


Genomic Information
GeneAllele 1Allele 2Protocol
Prkdcpoint mutation - transversionpoint mutation - transversionNot Specified


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