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Strain
FVB.129-Gt(ROSA)26Sortm1.1(rtTA2S*M2)Whsu Tg(tetO-Bmpr2*R899X)#Jwst

Summary Data Summary
Official Name FVB.129-Gt(ROSA)26Sortm1.1(rtTA2S*M2)Whsu Tg(tetO-Bmpr2*R899X)#Jwst
Common Name Rosa26R899X
Description The Bmpr2 was inserted into the Gt(ROSA)26 locus with a
point mutation R899X.

Rosa26-rtTA2 × TetO7-Bmpr2R899X FVB/N

ROSA mutation: A synthetic, modified version of the rtTA
gene preceded by a loxP flanked STOP sequence was inserted
into the Gt(ROSA)26Sor locus. Carriers were bred with mice
transgenic for cre recombinase under the control of beta
actin, excising the STOP sequence and leaving rtTA under the
endogenous Gt(ROSA)26Sor promoter. This version of rtTA
contains 5 amino acid changes in the TetR moiety (S12G,
E19G, A56P, D148E, and H179R) and a synthetic optimized
transactivating domain. This rtTA has reduced basal activity
and enhanced doxycycline sensitivity compared to wild-type
rtTA.

tetO transgene: A septad of tetracycline respons element
with a minimal CMV promoter drive inducible expression of
the mouse cDNA with nucleotide substitutions that result in
the amino acid substitution of a stop codon for arginine at
position 899 (R899X). The cDNA has a C-terminal FLAG tag and
is followed by the beta-globin intron and polyadenylation
sequence. The amino acid substitution does not alter SMAD
activity. No line number or name was provided (designated by
#).
Development Status Phenotyping ongoing
Creation Method transgenic
Breeding Type intercross
Phenotype Description Pulmonary hypertension, metabolic
derangements/hyperglycemia/weight gain
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Anna HemnesVanderbilt University


Genomic Information
GeneAllele 1Allele 2Protocol
Gt(ROSA)26Sorpoint mutation - transversionwildNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Hyperoxia synergizes with mutant bone morphogenic protein receptor 2 to cause metabolic stress, oxidant injury, and pulmonary hypertension.
Fessel JP, Flynn CR, Robinson LJ, Penner NL, Gladson S, Kang CJ, Wasserman DH, Hemnes AR, West JD
American journal of respiratory cell and molecular biology, 2013 (49), 778 - 787
23742019
Published

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