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Strain
C57BL/6N-Negr1tm1a(KOMP)Wtsi

Summary Data Summary
Official Name C57BL/6N-Negr1tm1a(KOMP)Wtsi
Common Name Negr1 (-/-)
Description The L1L2_Bact_P cassette was inserted at position 156858970
of Chromosome 3 upstream of the critical exon(s) (Build
GRCm38). The cassette is composed of an FRT site followed by
lacZ sequence and a loxP site. This first loxP site is
followed by neomycin under the control of the human
beta-actin promoter, SV40 polyA, a second FRT site and a
second loxP site. A third loxP site is inserted downstream
of the targeted exon(s) at position 156859924. The critical
exon(s) is/are thus flanked by loxP sites. A "conditional
ready" (floxed) allele can be created by flp recombinase
expression in mice carrying this allele. Subsequent cre
expression results in a knockout mouse. If cre expression
occurs without flp expression, a reporter knockout mouse
will be created.
Development Status Phenotyping ongoing
Creation Method knockout
Phenotype Description GWAS have identified several novel loci for obesity and body
mass-related traits. Ablation of NEGR1, neuronal growth
regulator 1, results in a small but steady reduction of body
mass. This study was designed to test the effect on energy
expenditure and body composition.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Ravi Kumar PasumarthiUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Negr1knockoutknockoutNot Specified


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