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Strain
C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J

Summary Data Summary
Official Name C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
Common Name C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
Description These Macrophage Fas-Induced Apoptosis (MAFIA) transgenic
mice have an inducible Fas suicide/ apoptotic system driven
by the mouse Csf1r, colony stimulating factor 1 receptor
promoter. The transgene insert contains a mutant human FK506
binding protein 1A, 12kDa (FKBP12) which preferentially
binds the dimerization drug AP20187. Enhanced Green
Fluorescent Protein (EGFP) fluorescence and transgene
expression is detected in 78% of isolated peritoneal cells.
EGFP fluorescence is variable among tissues (B- and T-cells
do not express EGFP). Administration of the dimerizing
reagent, AP20187, induces apoptosis in macrophages and
dendritic cells (intravenous injection of dimerizer is
recommended, since the intraperitoneal route can elicit
peritoneal adhesions). In treated mice, EGFP fluorescing
peritoneal and bone marrow macrophage numbers are depleted
by more than 90%, and macrophage numbers in blood, spleen,
lung and thymus by more than 70%. 7 days after cessation of
treatment, the EGFP fluorescing macrophage and dendritic
cell populations rebound. Homozygous mutant mice are viable,
fertile, normal in size and do not display any gross
physical or behavioral abnormalities. Prior to June 2008,
this strain may have contained multiple transgene insertion
sites (about which the donating investigator recommended
that all offspring be screened for EGFP expression [via flow
cytometry]). By June 2008, the live MAFIA mouse colony at
The Jackson Laboratory Repository was confirmed to have one
transgene integration site and retained the ability for
inducible macrophage deletion.
Development Status Phenotyping ongoing
Creation Method transgenic
Background C57BL/6
Phenotype Description No abnormal phenotype detected. Mice homozygous for the
transgene are viable and exhibit no gross physical or
behavioral abnormalities (MGI Ref ID J:89035)
TypeCount
Investigators 1
Genomics - Transgenes 3
Child Strains 1


Investigators
NameInstitution
Willa HsuehOhio State University-Main Campus


Genomic Information
EnhancerPromoterGeneCopies
Not SpecifiedCsf1rNgfrNot Specified
Not SpecifiedCsf1rFkbp1aNot Specified
Not SpecifiedCsf1rTnfrsf6Not Specified

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