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Strain
STOCK Hilpdatm1.1Nat/J

Summary Data Summary
Official Name STOCK Hilpdatm1.1Nat/J
Common Name Hig2 KO
Description LoxP sites were inserted on either side of the single coding
exon. The open reading frame for a membrane tethered human
placental alkaline phosphatase (hPLAP) was placed after the
second loxP site. An FRT-flanked neomycin selectable marker
used for ES cell selection (located just downstream of the
alkaline phosphatase reporter) was removed by flp-mediated
recombination.

The single exon of the 2310016C08Rik gene (commonly called
Hig2, hypoxia inducible gene 2; GenBank AF141311) is flanked
by loxP sites and followed by a membrane-tethered human
placental alkaline phosphatase (hPLAP) in this conditional
targeted mutation strain. Expression of the targeted gene is
expected to be normal in the conditional allele. Following
Cre-mediated recombination, the exon is excised enabling
transcription of hPLAP under the control of the Hig2
promoter. hPLAP is expressed only when the Hig2 coding
sequences are deleted by Cre-dependent excision. When
crossed with a germline Cre strain (Sox2-Cre), expression of
hPLAP was found to be widely expressed, with highest levels
in the vasculature.
Development Status Characterizing multiple lines
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 2


Investigators
NameInstitution
Michael CzechUniversity of Massachusetts Medical School


Genomic Information
GeneAllele 1Allele 2Protocol
2310016C08RikknockoutknockoutNot Specified


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