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Strain
B6.129-Slc2a4tm1Abel Tg(BGLAP-cre)1Clem

Summary Data Summary
Official Name B6.129-Slc2a4tm1Abel Tg(BGLAP-cre)1Clem
Common Name Glut4lox/lox Tg(BGLAP-cre)1Clem
Description The solute carrier family 2 (facilitated glucose
transporter) member 4 (Slc2a4 or Glut4) was floxed in the
strain. A transgene containing the bone gamma
carboxyglutamate protein (Bglap or Osteocalcin) promoter
driving the Cre gene was inserted to provide a conditional
knockout.

Glut4 was disrupted in the osteoblast by crossing
Glut4flox/flox mice maintained on a 129SV background with
osteocalcin-Cre transgenic mice maintained on a C57Bl/6
background.

Slc2a4 fl: LoxP sites were inserted flanking exon 10, which
encodes the external and internal glucose binding sites. A
neomycin selection cassette was also inserted downstream of
the final coding exon.

BGLAP-cre transgene: A human osteocalcin (BGLAP) promoter,
driving expression in osteoblasts, fused to a cre
recombinase gene was used for the transgene. The cre gene
was modified to include a nuclear localization signal and
human ACTB 3' untranslated region. Two lines were generated,
4 and 6. This allele represents line 6. Transgene expression
was observed in the calvaria, femur, and vertebrae by
Northern blot analysis.
Development Status Phenotyping ongoing
Creation Method knockin
Breeding Type intercross
Phenotype Description Disruption of Glut4 transporter in osteoblasts in vitro
eliminated insulin-stimulated glucose uptake, reduced
proliferation and diminished measures of osteoblast
maturation. Glut4 Ob-KO mice exhibited increased peripheral
adiposity in association with mild hyper-insulinemia but
marked insulin resistance measured by ITT.
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Publications 1


Investigators
NameInstitution
Thomas ClemensJohns Hopkins University School of Medicine


Genomic Information
GeneAllele 1Allele 2Protocol
Slc2a4knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedBglapCRENot Specified




PublicationAltmetricsSubmitted ByPubMed IDStatus
Glucose Transporter-4 Facilitates Insulin-Stimulated Glucose Uptake in Osteoblasts.
Li Z, Frey JL, Wong GW, Faugere MC, Wolfgang MJ, Kim JK, Riddle RC, Clemens TL
Endocrinology, 2016 (157), 4094 - 4103
Submitted Externally
27689415
Published

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