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Strain
C57BL/6-Ppargc1atm1Brsp Tg(Adipoq-cre)1Evdr

Summary Data Summary
Official Name C57BL/6-Ppargc1atm1Brsp Tg(Adipoq-cre)1Evdr
Common Name C57BL/6-Pgc1a-/- Tg(Adipoq-cre)1Evdr
Description Ppargc1a knockout mice were generated by crossing female
mice homozygous for the floxed allele with heterozygous
floxed male mice carrying a transgene expressing
cre-recombinase under control of the adiponectin promoter.
Mice carrying the floxed allele as well as mice carrying the
cre transgene were backcrossed 10 times to the C57BL/6CR
background.

To generate the Adipoq-Cre BAC transgenic mice, the 245 kb
RP23-90G21 bacterial artificial chromosome (BAC) containing
at least five mouse genes was modified by replacing the
starting ATG and 222 bp of the adiponectin gene with the
starting ATG and coding sequence of a Cre recombinase gene.
Founder line 1 was analyzed. These mice express Cre
recombinase effectively in white adipose tissue (WAT) and
brown adipose tissue (BAT), but not in macrophages
(including adipose-tissue resident macrophages, alveolar
macrophages, or thioglycollate-stimulated peritoneal
macrophages). The donating investigator reports highly
efficient Cre recombinase activity, with no ectopic
expression.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6Crl
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Bruce SpiegelmanHarvard University


Genomic Information
GeneAllele 1Allele 2Protocol
Ppargc1aknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedAdipoqCRENot Specified


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