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Strain
B6;129S4-Ptpn1tm2Bbk Tg(Cdh5-cre/ERT2)1Rha

Summary Data Summary
Official Name B6;129S4-Ptpn1tm2Bbk Tg(Cdh5-cre/ERT2)1Rha
Common Name B6;129S4-Ptpn1tm2Bbk/Mmjax Tg(Cdh5-cre/ERT2)1Rha
Description For the Ptpn1 floxed allele a targeting vector was designed
to place a loxP -flanked PGKneo cassette in intron 5 and a
loxP site in intron 8 encompassing the protein active site
and parts of the catalytic domain. The construct was
electroporated into 129S4/SvJae-derived J1 embryonic stem
(ES) cells. Correctly targeted ES cells were injected into
recipient blastocysts. The neomycin cassette was removed by
transient transfection with a Cre recombinase expressing
plasmid leaving loxP sites in introns 5 and 8.

The tamoxifen-inducible cre/ERT2 sequence was inserted into
a PAC (P1 artificial chromosome) containing the VE-cadherin
(Cdh5) gene to generate the transgene construct
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Michael SimonsYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Ptpn1knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedCdh5CRENot Specified


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