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Strain
B6(Cg)-Tfamtm1.1Ncdl Tg(CAG-cre/Esr1*)5Amc

Summary Data Summary
Official Name B6(Cg)-Tfamtm1.1Ncdl Tg(CAG-cre/Esr1*)5Amc
Common Name B6.Cg-Tfamtm1.1Ncdl Tg(CAG-cre/Esr1*)5Amc/J
Description A targeting vector was designed to insert a loxP site
upstream of Tfam exon 6, and a FRT-flanked neomycin
resistance (neo) cassette followed by a single loxP site
downstream of exon 7 of the gene. Flp mediated recombination
removed the neomycin cassette.

The pCAGGCre-ERTM transgene was designed with the CMV-IE
enhancer/chicken ß-actin/rabbit ß-globin hybrid promoter
(CAG; originally from the pCAGGS vector) and CreERTM fusion
gene (CreERTM; Cre recombinase fused to a G525R mutant form
of the mouse estrogen receptor ligand binding domain). The
transgene was introduced into B6CBF1 donor eggs. The
resulting male transgenic mice were first backcrossed for 2
generations on the SWR background. Next, mice were
backcrossed onto the C57BL/6J background for 5 generations.

Tfamflox/flox mice were bred to ERCre transgenic mice from
Jackson (stock
# 004682) for inducible, 4OHT-mediated deletion.
Development Status Phenotyping ongoing
Creation Method knockout
Background C57BL/6
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Gerald ShadelYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Tfamknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedACTBEsr1Not Specified


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