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Strain
B6.129S-Itpr2tm1.1Chen

Summary Data Summary
Official Name B6.129S-Itpr2tm1.1Chen
Common Name B6.129S-Itpr2-/-
Description Itpr2 genomic DNA was isolated from a 129SVJ mouse genomic
library and used to construct an Itpr2 targeting vector
containing a neomycin selection cassette flanked by FRT
sites. The targeting vector was linearized with Notl and
electroporated into R1 embryonic stem cells. Male chimeras
were bred with female Black Swiss mice to generate germline
transmitted floxed heterozygous mice (Itpr2+/flox) which
were intercrossed and crossed with Pro-Cre mice, which
restricted Cre expression to male germ cells undergoing
spermatogenesis, to generate mice heterozygous for Itpr2
floxed allele and Pro-Cre. Pro-Cre, Itpr2+/- flox males were
crossed to female breeders to generate germline heterozygous
null mutant hets (Itpr2+/-) which were subsequently
intercrossed.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Colleen FeriodYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Itpr2knockoutknockoutNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Inositol 1,4,5-trisphosphate receptor type II (InsP3R-II) is reduced in obese mice, but metabolic homeostasis is preserved in mice lacking InsP3R-II.
Feriod CN, Nguyen L, Jurczak MJ, Kruglov EA, Nathanson MH, Shulman GI, Bennett AM, Ehrlich BE
American journal of physiology. Endocrinology and metabolism, 2014 (307), E1057 - E1064
25315698
Published

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