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Strain
C57BL/6J-Tg(Ckmm-Aspscr1)1Bog/+

Summary Data Summary
Official Name C57BL/6J-Tg(Ckmm-Aspscr1)1Bog/+
Common Name C57BL/6J-Tg(Ckmm-Aspscr1)1Bog/+
Description Transgenic mTUGUBX-Cter mice were generated by cloning the
cDNA sequence for a truncated murine TUG protein, UBX-Cter
(residues 377–550), after a 1.3-kb segment derived from the
mouse muscle creatine kinase promoter (-1256MCK-3Emut) (28,
29). This promoter (termed MCK3E) contains specific
mutations to drive expression in skeletal muscle but not in
cardiac muscle or other tissues (30, 31). Following the
coding sequence, a 2.2-kb segment of the human growth
hormone gene was used to provide splice sites and
polyadenylation and termination signals. The transgene was
microinjected into SJL × C57BL/6 F2 fertilized oocytes,
which were implanted in pseudopregnant females. Founder pups
were identified by PCR of tail biopsies for a 171-bp segment
of the human growth hormone sequence. Of 12 transgenic lines
obtained, three were selected for further analysis.
Preliminary analyses indicated that the phenotype was most
robust in the line with greatest expression of the
transgene, and this line was backcrossed at least 10
generations onto C57BL/6 (The Jackson Laboratory) and used
in the heterozygous state for all analyses presented here.
Development Status Phenotyping ongoing
Creation Method transgenic
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Jonathan BoganYale University


Genomic Information
EnhancerPromoterGeneCopies
Not SpecifiedCkmmAspscr1Not Specified


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