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Strain
B6N.129S2-Casp1tm1Flv/J

Summary Data Summary
Official Name B6N.129S2-Casp1tm1Flv/J
Common Name B6N.129S2-Casp1tm1Flv/J
Description A 2.5 kb EcoRI-HindIII fragment containing caspase 1 exons
4-6 and a 1.3 kb EcoRI-SmaI fragment containing exons 8-10
were subcloned with the neomycin resistance gene cassette in
a thymidine kinase gene-expressing plasmid to generate the
targeting vector. The vector was linearized and introduced
into 129S2/SvPas-derived embryonic stem (ES) cells by
electroporation. 63 clones resistant to G418 and Gancyclovir
were screened by PCR using and exon 10 primer and a neo
cassette specific primer. One correctly targeted clone was
confirmed by Southern blot analysis. Chimeric mice were
generated from the targeted ES cells and chimeras bred to
germline. The donating investigator reported that the
progeny were backcrossed at least 10 generation on C57BL/6N
background
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
Phenotype Description Caspase 1 (also known as interleukin-1beta converting enzyme
or ICE) knockout mice lack part of exons 6 and 7 of the
Casp1 gene and do not process pro-IL-1beta (IL1B) or
pro-IL-18 (IL18) into their mature forms. Therefore,
stimulation of CASP1-deficient monocytes by activators of
multiple pattern recognition receptors that form
inflammasomes with CASP1 (including Nlrp3 and Nlrc4) fail to
secrete mature IL1B or IL18. In addition, secretion of
IL-1alpha (IL1A) is reduced. Caspase-1 is involved in
particular cell death pathways including pyroptosis, and
caspase-1 deficient cells demonstrate reduced lysis upon
infection with certain pathogens.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Richard FlavellYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Casp1knockoutknockoutNot Specified


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