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Strain
B6.Cg-Pkd2tm2Som Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-cre)1Jaw

Summary Data Summary
Official Name B6.Cg-Pkd2tm2Som Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-cre)1Jaw
Common Name Pkd2tm2Som Tg(Pax8-rtTA2S*M2)1Koes Tg(TetO-Cre)
Description Pkd2 KO: Exon 1 was disrupted by the insertion of a
selectable Neo cassette in the same transcriptional
orientation as the gene. This allele is a a presumed true
null as opposed to WS25 which is an apparent hypomorphic
allele.

Pax8 TG: The Pax8-rtTA construct contains an optimized rtTA
variant (rtTA2s-M2) cDNA and SV40 polyA sequence replaced at
the endogenous ATG translational start site of a murine Pax8
sequence. The rtTA2S*M2 variant contains 5 amino acid
changes in the TetR moiety (S12G, E19G, A56P, D148E, and
H179R) and a synthetic optimized transactivating domain,
resulting in reduced basal activity and enhanced doxycycline
sensitivity compared to wild-type rtTA.

TetO TG: The transgene is composed of 7 tetO sites, a
minimal CMV promoter, and sequence encoding cre recombinase.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1
Publications 1


Investigators
NameInstitution
Xin TianYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Pkd2knockoutknockoutNot Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Loss of cilia suppresses cyst growth in genetic models of autosomal dominant polycystic kidney disease.
Ma M, Tian X, Igarashi P, Pazour GJ, Somlo S
Nature genetics, 2013 (45), 1004 - 12
Submitted Externally
23892607
Published

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