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Strain
B6.129P2-Lyz2tm1(cre)Ifo Hsp90b1tm1Zhli

Summary Data Summary
Official Name B6.129P2-Lyz2tm1(cre)Ifo Hsp90b1tm1Zhli
Common Name Lyz2tm1(cre)Ifo Hsp90b1tm1Zhli
Description Gp96 conditional deficient mice were generated by homologous
recombination. In brief, a 17 kb mouse genomic DNA fragment
was cloned from the mouse 129Sv/Ev lambda genomic library.
This genomic fragment contained exon 1–4 of Hsp90b1. The
targeting vector was constructed to insert the Neo gene
cassette (flanked by loxP sites) before the exon 1
(containing ATG). The third loxP site was inserted 230 bp
downstream of exon 1. A HSV-tk cassette was introduced at
the 3' end of the genomic fragment (Figure 1A).
10 µg of the targeting vector was linearized by NotI and
then transfected by electroporation of 129/SvEv embryonic
stem (ES) cells. After double selection in G418 and
gancyclovir, 250 surviving colonies were expanded for PCR
analysis to identify recombinant clones. The correctly
targeted ES cell line was microinjected into the C57BL/6J
blastocysts and produced four female chimeras (100% agouti).
The chimeric mice were then mated to generate germline
transmission of the Hsp90b1flox mice. These were then bred
to LysMCre mice purchase from Jackson Laboratory.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 2
Experiments 1


Investigators
NameInstitution
Saleh RachidiMedical University of South Carolina


Genomic Information
GeneAllele 1Allele 2Protocol
Lyz2transgenicwildNot Specified
Hsp90b1knockin (lox)knockin (lox)Not Specified


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