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Strain
B6.Cg-Ldlrtm1Her Tg(MSR1-CES1D)1Gho

Summary Data Summary
Official Name B6.Cg-Ldlrtm1Her Tg(MSR1-CES1D)1Gho
Common Name LDLR-/- CEHTg
Description The human scavenger receptor (SR-A) promoter/enhancer was
used to drive macrophage-specific expression of human CEH in
mice. The plasmid pAL1 containing the SR-A promoter/enhancer
and all the necessary elements for chimeric transgene
construction (poly linker region and heterologous intron)
was obtained from Christopher Glass (UCSD, La Jolla,
California, USA). Full-length human CEH cDNA (~2 kb) with an
inframe stop codon was cloned into the EcoRI site in the
poly-linker region of the plasmid pAL1. The sequence of the
chimeric transgene was confirmed by sequencing. The SRA-CEH
chimeric transgene was separated from the vector sequence by
digestion with NotI and XhoI, purified by agarose gel
electrophoresis, and injected into the pronuclei of
fertilized mouse eggs obtained from superovulated female
mice (C57BL/6 or Balb-c/C57BL/6 hybrids). The injected eggs
were surgically transferred to oviducts of surrogate
females.

To evaluate the role of CEH in the development of
atherosclerosis, CEH-transgenic mice were crossed with
atherosclerosis-susceptible Ldlr–/– mice that had a C57BL/6
background (The Jackson Laboratory).
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1
Publications 1


Investigators
NameInstitution
Shobha GhoshVirginia Commonwealth University


Genomic Information
GeneAllele 1Allele 2Protocol
LdlrknockoutknockoutNot Specified
EnhancerPromoterGeneCopies
Not SpecifiedMsr1Ces1Not Specified






PublicationAltmetricsSubmitted ByPubMed IDStatus
Macrophage-specific transgenic expression of cholesteryl ester hydrolase significantly reduces atherosclerosis and lesion necrosis in Ldlr mice.
Zhao B, Song J, Chow WN, St Clair RW, Rudel LL, Ghosh S
The Journal of clinical investigation, 2007 (117), 2983 - 92
Submitted Externally
17885686
Published

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