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Strain
C57BL/6-Fasntm1Sem Tg(Ucp1-cre)1Evdr

Summary Data Summary
Official Name C57BL/6-Fasntm1Sem Tg(Ucp1-cre)1Evdr
Common Name Ucp1-cre Fasn KO
Description Fasn tm1Sem: A loxP flanked neo cassette was placed upstream
of exon 4 and a third loxP site was placed downstream of
exon 8, of the Fasn (fatty acid synthase) gene. Insertion of
targeting vector was verified by Southern blotting.

Tg(Ucp1-cre)1Evdr: The C57BL/6 mouse bacterial artificial
chromosome (BAC) 148M1 containing the entire Ucp1 gene, and
other genes, was modified by inserting sequence encoding cre
recombinase, into the translation initiation site into exon
1 of the Ucp1 gene. An FRT site flanked KAN cassette and a
loxP site were removed. DNA from the modified BAC clone was
used as the source for the transgene, which was
microinjected into the pronucleus of FVB fertilized eggs. A
founder line was subsequently established.
Development Status Phenotyping ongoing
Creation Method knockout
Phenotype Description FASNKO mice survive the cold but may have reduced fatty acid
oxidation and changes in fuel use, as compared to wild type
control mice.
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1


Investigators
NameInstitution
Michael CzechUniversity of Massachusetts Medical School


Genomic Information
GeneAllele 1Allele 2Protocol
Fasnknockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedUcp1CRENot Specified

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