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Strain
B6.129S6-Rag2tm1Fwa

Summary Data Summary
Official Name B6.129S6-Rag2tm1Fwa
Common Name B6.129S6-Rag2tm1Fwa N12
Description Rag2: A neomycin resistance cassette replaced a 0.85kb
segment of the open reading frame, eliminating the encoding
of 286 of the 527 amino acids of the protein.

The Rag2 mouse was developed in the laboratory of Frederick
W. Alt at Columbia University. The model was created by
targeting the Rag2 gene in CCE ES cells and injecting the
targeted cells into blastocysts. This line originated from
model RAG2-M on a 129S6/SvEv background. The mice were
backcrossed twelve generations (N12) to a C57BL/6NTac inbred
background. Heterozygotes were intercrossed to generate
homozygous targeted mutation mice. The line is maintained by
incrossing homozygous mice.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 2


Investigators
NameInstitution
William MurphyUniversity of California - Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Rag2knockoutknockoutNot Specified


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