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Strain
C57BL/6NJ-Crattm1.1Pbrc Sirt3tm1.1Auw Tg(Ckmm-cre)5Khn

Summary Data Summary
Official Name C57BL/6NJ-Crattm1.1Pbrc Sirt3tm1.1Auw Tg(Ckmm-cre)5Khn
Common Name Cratfl Sirt3L2 MCK-cre DKO
Description Crat: A loxP site was inserted into intron 8 and a floxed
neo cassette was inserted into intron 11 via homologous
recombination. Cre mediated recombination removed the neo
cassette.

Sirt3: The targeting vector is designed to insert loxP sites
flanking exons 2-3 of the gene, and a FRT-flanked neomycin
selection cassette was inserted upstream of exon 2.
Flp-mediated recombination removed the FRT-flanked neo
cassette.

MCK-cre: A 6.5 kb genomic DNA fragment of the Ckmm gene
containing the promoter and enhancer 1, untranslated exon 1,
3kb of intron 1 including the enhancer 2 region, and the
first 16 bp of exon 2 drives expression of a modified cre
with an SV40 large T antigen nuclear localization signal.
Expression is directed to the heart and skeletal muscle.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Modifications 2
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Ashley WilliamsDuke University Medical Center


Genomic Information
GeneAllele 1Allele 2Protocol
Cratknockin (lox)knockin (lox)Not Specified
Sirt3knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedCkmmCRENot Specified


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